Research Centers
Permanent URI for this communityhttps://scholarworks.montana.edu/handle/1/13
The Department of Research Centers was created in 1994. The Department represents the faculty and staff that conduct research and outreach programs at 7 off-campus research centers.
Central Agricultural Research Center
Eastern Agricultural Research Center
Northern Agricultural Research Center
Northwestern Agricultural Research Center
Southern Agricultural Research Center
Western Agricultural Research Center
Western Triangle Ag Research Center
Central Agricultural Research Center
Eastern Agricultural Research Center
Northern Agricultural Research Center
Northwestern Agricultural Research Center
Southern Agricultural Research Center
Western Agricultural Research Center
Western Triangle Ag Research Center
Browse
6 results
Search Results
Item Compositional profiling of the rhizosphere microbiome of Canada thistle reveals consistent patterns across the United States northern Great Plains(Springer Science and Business Media LLC, 2024-08) Eberly, Jed O.; Hurd, Asa; Oli, Dipiza; Dyer, Alan T.; Seipel, Tim F.; Carr, Patrick M.Canada thistle is a pervasive perennial weed, causing challenges to agricultural and natural ecosystems globally. Although research has focused on the phenology, genetics, and control of Canada thistle, little is known about the rhizosphere microbiome and the role plant–microbe interactions play in invasion success. This study investigated the rhizosphere microbiome of Canada thistle across diverse climates, soils, and crops in the U.S. northern Great Plains. Soil and rhizosphere samples were collected and bacterial 16S and fungal ITS2 sequencing were performed to characterize the core microbiome and identify potential factors contributing to invasion success. Amplicon sequencing revealed a stable core microbiome that was detected in the Canada thistle rhizosphere across all locations. The core microbiome was dominated by the bacterial phyla Actinobacteriota and Proteobacteria and fungal phyla Ascomycota and Basidiomycota. Differential abundance analysis showed rhizosphere fungal communities were enriched in pathogen-containing genera with a 1.7-fold greater abundance of Fusaria and a 2.6-fold greater abundance of Gibberella compared to bulk soil. Predictive functional profiling showed rhizosphere communities were enriched (p < 0.05, FDR corrected) in plant pathogen fungal guilds which represented 19% of the fungal community. The rhizosphere microbiome was similar in composition across environments, highlighting the stable association between Canada thistle and specific microbial taxa. This study characterized the core microbiome of Canada thistle, and the findings highlight plant–microbe interactions shaping invasive behavior. These findings are important for understanding the ecological impacts of plant invasion and soil-microbe ecological processes.Item Compositional profiling of the rhizosphere microbiome of Canada thistle reveals consistent patterns across the United States northern Great Plains(Springer Science and Business Media LLC, 2024) Eberly, Jed O.; Hurd, Asa; Oli, Dipiza; Dyer, Alan T.; Seipel, Tim F.; Carr, Patrick M.Canada thistle is a pervasive perennial weed, causing challenges to agricultural and natural ecosystems globally. Although research has focused on the phenology, genetics, and control of Canada thistle, little is known about the rhizosphere microbiome and the role plant–microbe interactions play in invasion success. This study investigated the rhizosphere microbiome of Canada thistle across diverse climates, soils, and crops in the U.S. northern Great Plains. Soil and rhizosphere samples were collected and bacterial 16S and fungal ITS2 sequencing were performed to characterize the core microbiome and identify potential factors contributing to invasion success. Amplicon sequencing revealed a stable core microbiome that was detected in the Canada thistle rhizosphere across all locations. The core microbiome was dominated by the bacterial phyla Actinobacteriota and Proteobacteria and fungal phyla Ascomycota and Basidiomycota. Differential abundance analysis showed rhizosphere fungal communities were enriched in pathogen-containing genera with a 1.7-fold greater abundance of Fusaria and a 2.6-fold greater abundance of Gibberella compared to bulk soil. Predictive functional profiling showed rhizosphere communities were enriched (p < 0.05, FDR corrected) in plant pathogen fungal guilds which represented 19% of the fungal community. The rhizosphere microbiome was similar in composition across environments, highlighting the stable association between Canada thistle and specific microbial taxa. This study characterized the core microbiome of Canada thistle, and the findings highlight plant–microbe interactions shaping invasive behavior. These findings are important for understanding the ecological impacts of plant invasion and soil-microbe ecological processes.Item Changes in Biological Soil Health Properties in Response to Increased Crop Diversity in a Dryland Wheat-Based Cropping System(Informa UK Limited, 2024-08) Eberly, Jed O.; Hammontree, Jenni W.; Fordyce, Simion I.; Jones, Clain A.; Carr, Patrick M.Diversifying wheat (Triticum aestivum L.)-based cropping systems is important for the sustainability of dryland agriculture. Research has focused on the agronomic benefits of increased crop diversity in semi-arid environments, but less is known about the impacts of increased crop diversity on the soil microbial community. This work compared soil health parameters between a continuous wheat crop sequence to a diverse sequence that included pea (Pisum sativum L.), proso millet (Panicum miliaceum L.), safflower (Carthamus tinctorius, L.), and spring wheat. Respiration was higher (p < .005) in the diverse sequence while activity of N-acetyl-β-d-glucosaminidase was lower (p < .05) with a mean rate of 26.3 and 16.3 mg ρ-nitrophenol kg−1 soil h−1 for the continuous wheat and diverse sequences, respectively. The mean net nitrogen mineralized during the growing season was 33.2 ± 2.5 kg ha−1 and was not different between treatments (p > .05). No difference was observed in bacterial alpha diversity, while fungal community diversity was 52% lower in the diverse rotation. The results of this work suggest that specific crops in a rotation may impact microbial processes related to nitrogen mineralization and that the soil fungal community may be more sensitive to changes in crop sequence than the soil bacterial community.Item Sentinel-2-based predictions of soil depth to inform water and nutrient retention strategies in dryland wheat(Elsevier BV, 2023-11) Fordyce, Simon I.; Carr, Patrick M.; Jones, Clain; Eberly, Jed O.; Sigler, W. Adam; Ewing, Stephanie; Powell, Scott L.The thickness or depth of fine-textured soil (zf) dominates water storage capacity and exerts a control on nutrient leaching in semi-arid agroecosystems. At small pixel sizes (< 1 m; ‘fine resolution’), the normalized difference vegetation index (NDVI) of cereal crops during senescence (Zadoks Growth Stages [ZGS] 90–93) offers a promising alternative to destructive sampling of zf using soil pits. However, it is unclear whether correlations between zf and NDVI exist (a) at larger pixel sizes (1–10 m; ‘intermediate resolution’) and (b) across field boundaries. The relationship of zf to NDVI of wheat (Triticum aestivum L.) was tested using images from a combination of multispectral sensors and fields in central Montana. NDVI was derived for one field using sensors of fine and intermediate spatial resolution and for three fields using intermediate resolution sensors only. Among images acquired during crop senescence, zf was correlated with NDVI (p < 0.05) independent of sensor (p = 0.22) and field (p = 0.94). The zf relationship to NDVI was highly dependent on acquisition day (p < 0.05), but only when pre-senescence (ZGS ≤ 89) images were included in the analysis. Results indicate that cereal crop NDVI of intermediate resolution can be used to characterize zf across field boundaries if image acquisition occurs during crop senescence. Based on these findings, an empirical index was derived from multi-temporal Sentinel-2 imagery to estimate zf on fields in and beyond the study area.Item Cover crops to improve soil health in the North American Great Plains(Wiley, 2021-09) Obour, Augustine K.; Simon, Logan M.; Holman, Johnathon D.; Carr, Patrick M.; Schipanski, Meagan; Fonte, Steven; Ghimire, Rajan; Nleya, Thandiwe; Blanco‐Canqui, HumbertoRotating cereal crops (e.g., wheat [Triticum aestivum L.] with a 10- to 21-mo summer fallow period [fallow]) is a common farming practice in dryland (rainfed) agricultural regions. Fallow is associated with several challenges including low precipitation storage efficiency, depletion of soil organic carbon (SOC), loss of soil fertility, little crop residue retention and soil erosion, and few control options for herbicide-resistant (HR) weeds. The inability to effectively control HR weeds poses a major challenge to maintaining soil and water conservation practices such as no-tillage, as some producers are considering tillage to control weeds. Cover crop (CC) integration into wheat-based production systems to replace portions of the fallow period provides an opportunity to increase SOC, improve soil fertility, suppress weeds, and increase profitability of dryland crop production, especially when CCs are used as forage. This forum paper used the North American Great Plains as a model region to review information on (a) challenges of dryland agriculture; (b) integrating CCs in dryland agriculture; (c) benefits, challenges, and limitations of CCs in dryland crop production; (d) management options for CC integration in dryland grain systems; and (e) recommendations for future research efforts.Item Evaluation of environment and cultivar impact on lentil protein, starch, mineral nutrients, and yield(Wiley, 2021-12) Chen, Chengci; Etemadi, Fatemeh; Franck, William; Franck, Sooyoung; Abdelhamid, Magdi T.; Ahmadi, Jafar; Mohammed, Yesuf Assen; Lamb, Peggy F.; Miller, John H.; Carr, Patrick M.; McPhee, Kevin; Zhou, Yi; Torabian, Shahram; Qin, RuijunLentil (Lens culinaris Medik.) is an important source of protein, starch, and mineral nutrients in many parts of the world. However, the impact of environment and cultivar on the enrichment of these nutrients is not well understood. Four lentil cultivars (‘Avondale’, ‘CDC Richlea’, ‘CDC Maxim’, and ‘CDC Imvincible’) varying in color, seed size, and maturity were evaluated at five Montana locations with diverse climatic and soil conditions over 3 yr. Significant cultivar, location, and year effects were found for yield, protein, starch, and minerals. Grain protein concentration was the highest at Moccasin (262 g kg−1) and lowest at Richland (246 g kg−1), whereas starch concentration was the highest at Richland (455 g kg−1) and lowest at Moccasin(441gkg−1). Among cultivars, Avondale was the top yielding cultivar (1965 kg ha−1)and adaptable to most of the environments; CDC Imvincible was the top protein producer (265 g kg−1); and CDC Richlea is the leading starch producer (456 g kg−1). Grain protein concentration was negatively correlated with starch. Lentil grains varied in nutrient concentrations across locations, with the north central Montana region producing 10- to 20-times greater selenium concentration than other locations. CDC Maxim had the highest iron (62.1 mg kg−1) and zinc (31.5 mg kg−1) concentrations.Seed protein concentration was positively correlated with phosphorus, sulfur, cop-per, and boron. Seed starch is positively correlated with magnesium and manganese.Results suggest that plant breeding and production site selection could enrich lentil nutrient concentrations to help combat malnutrition in the world.