Research Centers
Permanent URI for this communityhttps://scholarworks.montana.edu/handle/1/13
The Department of Research Centers was created in 1994. The Department represents the faculty and staff that conduct research and outreach programs at 7 off-campus research centers.
Central Agricultural Research Center
Eastern Agricultural Research Center
Northern Agricultural Research Center
Northwestern Agricultural Research Center
Southern Agricultural Research Center
Western Agricultural Research Center
Western Triangle Ag Research Center
Central Agricultural Research Center
Eastern Agricultural Research Center
Northern Agricultural Research Center
Northwestern Agricultural Research Center
Southern Agricultural Research Center
Western Agricultural Research Center
Western Triangle Ag Research Center
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56 results
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Item Characterization of resistance to Cephus cinctus Norton (Hymenoptera: Cephidae) in barley germplasm(2018-04) Varella, Andrea C.; Talbert, Luther E.; Achhami, Buddhi B.; Blake, Nancy K.; Hofland, Megan L.; Sherman, Jamie D.; Lamb, Peggy F.; Reddy, Gadi V. P.; Weaver, David K.Most barley cultivars have some degree of resistance to the wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae). Damage caused by WSS is currently observed in fields of barley grown in the Northern Great Plains, but the impact of WSS damage among cultivars due to genetic differences within the barley germplasm is not known. Specifically, little is known about the mechanisms underlying WSS resistance in barley. We characterized WSS resistance in a subset of the spring barley CAP (Coordinated Agricultural Project) germplasm panel containing 193 current and historically important breeding lines from six North American breeding programs. Panel lines were grown in WSS infested fields for two consecutive years. Lines were characterized for stem solidness, stem cutting, WSS infestation (antixenosis), larval mortality (antibiosis), and parasitism (indirect plant defense). Variation in resistance to WSS in barley was compared to observations made for solid-stemmed resistant and hollow-stemmed susceptible wheat lines. Results indicate that both antibiosis and antixenosis are involved in the resistance of barley to the WSS, but antibiosis seems to be more prevalent. Almost all of the barley lines had greater larval mortality than the hollow-stemmed wheat lines, and only a few barley lines had mortality as low as that observed in the solid-stemmed wheat line. Since barley lines lack solid stems, it is apparent that barley has a different form of antibiosis. Our results provide information for use of barley in rotation to control the WSS and may provide a basis for identification of new approaches for improving WSS resistance in wheat.Item Entomopathogens in conjunction with imidacloprid could be used to manage wireworms (Coleoptera: Elateridae) on spring wheat(2018-02) Antwi, Frank B.; Shrestha, Govinda; Reddy, Gadi V. P.; Jaronski, Stefan T.We examined the effect of biopesticides used alone, mixed with other biopesticides, or in conjunction with an imidacloprid against wireworms (Coleoptera: Elateridae) in spring wheat Triticum aestivum Linnaeus (Poaceae) (variety: Duclair). The study was conducted at Ledger and Valier, Montana, United States of America in 2015 and 2016. Ten biopesticides (spinosad, azadirachtin, pyrethrin, Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Fungi: Clavicipitaceae) GHA (Mycotrol), B. bassiana ANT-03 (BioCeres), Chromobacterium subtsugae Martin et al. (Bacteria: Neisseriaceae), Burkholderia Yabuuchi et al. (Burkholderiaceae) species, Metarhizium brunneum Petch (Fungi: Clavicipitaceae) ESC1 (MbESC1), and M. brunneum F52 (MetF52) as microsclerotial and corn grit-based granules) were tested in addition to thimet and imidacloprid. Treatment efficacy was based on plant stand protection, wireworm populations, and yield. In 2015, there was considerable variation between sites in treatment efficacy. Mycotrol, BioCeres, MetF52+spinosad, and MetF52+imidacloprid applications protected seedlings from wireworm damage better than the control at Ledger, while only MetF52 at Valier. Wireworm populations were significantly higher with Mycotrol, spinosad, MetF52+spinosad, MbESC1 (25 g/L), and MetF52 treatments, compared with control, at 14 and 28 days post application at Ledger, but without effect at Valier, 2015. We found significantly higher yield in plots treated with imidacloprid+MetF52 and Mycotrol+azadirachtin (Xpulse) compared with control at Ledger. In 2016, no significant treatment effects were observed at either site. In summary, this study provides insight on what treatments should be explored in more detail despite variable results.Item Internode morphometrics and allometry of Tonkin Cane Pseudosasa amabilis(2018-11) Cheng, Liang; Cang, Hui; Reddy, Gadi V. P.; Yu-Long, Ding; Pei-Jian, ShiPseudosasa amabilis (McClure) (Poales: Gramineae) is a typical bamboo species naturally distributed in large area of south China and famous for its culm strength. Although bamboos were found to share the same development rule, the detailed internode morphology of bamboo culm was actually not fully expressed. We explored internode morphology of P. amabilis using 11 different physical parameters in different dimensions (1–4). As Taylor's power law (TPL) is generally applicable to describe relationship between mean and variance of population density, here we used TPL to evaluate the differences between internodes, and further, the relationship between dimension and TPL. Results showed that length (L), hollow radius (HR), hollow area (HA), hollow cylinder volume (HCV), total cylinder volume (TCV), density (De), and weight (W) all presented positive skewed distribution in varying degrees. For the basic one‐dimensional parameters, the 9th internode was the longest, the 7th the heaviest, while thickness (T) decreased with internodes. Diameter (D) decreased in general but with an inconspicuous local mode at the 5–6th internodes, potentially due to the rapid height growth. The longest (9th) internode was the “turning point” for T‐D and HR‐D relationships. Scatter plot changing trends of W to the one‐dimensional parameters after the heaviest (7th) internode were reversed, indicating a deceleration of growth speed. TPL was not holding well in one‐dimensional parameters (R2: 0.5413–0.8125), but keep increasing as the parameter's dimension increasing (R2 > 0.92 for two‐dimensional, R2 > 0.97 for three‐dimensional, and R2 > 0.99 for four‐dimensional parameters.), suggesting an emergence mechanism of TPL related to both the physical dimensions of morphological measures and the allometric growth of bamboo. From the physical fundamental level, all existences are the expression of energy distribution in different dimensions, implying a more general rule that energy distribution holds better TPL in higher dimension level.Item Field efficacy of insect pathogen, botanical and jasmonic acid for the management of wheat midge Sitodiplosis mosellana the impact on adult parasitoid ... populations in spring wheat(2017-10) Shrestha, Govinda; Reddy, Gadi V. P.The wheat midge, Sitodiplosis mosellana, is a serious pest of wheat worldwide. In North America, management of S. mosellana in spring wheat relies on the timely application of pesticides, based on midge adults levels caught in pheromone traps or seen via field scouting during wheat heading. In this context, biopesticides can be an effective alternative to pesticides for controlling S. mosellana within an integrated pest management program. A field study using insect pathogenic fungus Beauveria bassiana GHA, nematode Steinernema feltiae with Barricade polymer gel 1 %, pyrethrin, combined formulations of B. bassiana GHA and pyrethrin, Jasmonic acid (JA) and chlorpyrifos (chemical check) was performed to determine to which extent they affect midge larval populations, kernel damage levels, grain yield and quality, and the impacts on adult parasitoid Macroglenes penetrans populations. The results indicated that biopesticides JA and S. feltiae were the most effective in reducing larval populations and kernel damage levels, and produced a higher spring wheat yield when compared to the water control at both study locations (East Valier and North Valier, Montana, USA). Increased test weight in wheat had been recorded with two previous biopesticides at East Valier but not for North Valier, when compared over water control. These results were comparable in efficacy to the chlorpyrifos. The present study also suggested that B. bassiana and pyrethrin may work synergistically, as exemplified by lower total larval populations and kernel damage levels when applied together. This study did not demonstrate the effect of any treatments on M. penetrans populations.Item Evaluation of trap crops for the management of wireworms in spring wheat in Montana(2017-12) Adhikari, Ashish; Reddy, Gadi V. P.The polyphagous larvae of click beetles (Coleoptera: Elateridae) are major pests of spring wheat in Montana, USA. Presently available insecticides are unable to provide control over wireworm populations, and the use of natural enemies has not been successful under field conditions. In this study, we examined the effect of seven trap crops: pea, lentil, canola, corn, durum, barley, and wheat, for their attractiveness to wireworms compared to spring wheat. Experimental plots were located in two commercial grain fields in Valier and Ledger, Montana, USA and the trials took place from May to August in 2015 and 2016. Wheat plants damaged by wireworms were recorded and their relative locations in wheat rows and adjacent trap crop rows within a plot were determined using destructive soil samples. In 2016, variable row spacing (0.25, 0.5, 0.75, and 1 m) between the trap crops (pea and lentil) and wheat was assessed. Shade house bioassays were conducted using potted pea, lentil, and wheat plants to support field trial results. Limonius californicus larvae, released at the center of each pot were sampled 4 and 10 days after sowing. Wheat intercropped with pea and lentil had significantly fewer damaged wheat plants. Wireworm numbers were lower in wheat intercropped with pea compared to the control for both locations and years. Shade house results corresponded with field results, with more wireworms collected from pea and lentil than wheat. In the spacing trials, wheat plant counts were also significantly higher when paired with pea and lentil, particularly at 0.5 m spacing. Regardless of inter-row spacing, significantly fewer wireworms were associated with wheat when intercropped with pea and lentil trap crops.Item Effects of Irradiation Dose on Sperm Production, Insemination, and Male Mating Possible Period in the Sweetpotato Weevil (Coleoptera: Brentidae)(2018-05) Hiroyoshi, Satoshi; Mistunaga, Takayuki; Kohama, Tsuguo; Reddy, Gadi V. P.The sterile insect technique (SIT) has been used for the control or eradication of target insect pests. To successfully apply SIT, it is very important to clarify the effect of irradiation on male reproduction in the target pest, because their mating and spermiogenesis abilities affect the success of eradication program. The sweetpotato weevil, Cylas formicarius (Fabricius) (Coleoptera: Brentidae) is a notorious and worldwide pest of sweet potato. We investigated the effect of irradiation at five doses ranging from 0 (control) to 150 Gy on 9-d-old males. Survival rate of the control (no treatment) remained high from day 10 to 20 of adult life, whereas higher doses of irradiation reduced it, maximally by approximately 70%. Mating rates showed a similar tendency. Radiation dose neither affected sperm production nor sperm transfer at any dose, although spermiogenesis is active during the adult stage. However, radiation dose affected the lifetime total of ejaculated sperm number, likely because of fewer matings by irradiated males. These results suggest that use of a dose of 150 Gy or higher is appropriate for the final step of eradication of this weevil. At least, lower dose of irradiation may arise the inadequate sterilization, resulting in a failure of eradication program. We conclude that weekly release of sweetpotato weevil sterilized with high dose, achieving complete sterilization, could be useful for eradication program after reducing the population by male annihilation method.Item Potential biopesticides for crucifer flea beetle, Phyllotreta cruciferae (Coleoptera: Chrysomelidae) management under dryland canola production in Montana(2018-04) Shabeg, Briar Singh; Antwi, Frank; Shrestha, Govinda; Sharma, Anamika; Reddy, Gadi V. P.The crucifer flea beetle, Phyllotreta cruciferae (Goeze), is an economically important and dominant pest of canola (Brassica napus L) in the Northern Great Plains of the USA. The current flea beetle management strategy is based on using synthetic chemical treated seeds and if necessary, foliar spray of chemicals at canola seedlings in early spring for targeting adult population. However, there is an increasing demand for development of alternative management strategies for P. cruciferae pertaining to concerns over the development of resistance to synthetic insecticides and non-target effects on pollinators and other beneficial insects. Replicated field trials were conducted to test the efficacy of several commercially available biopesticides including Entrust® (spinosad), entomopathogenic nematode Steinernema feltiae + Barricade® (polymer gel 1%), Aza-Direct® (azadirachtin), Pyganic 1.4® EC (pyrethrin), Grandevo® SC (Chromobacterium subtsugae), Venerate® XC (Heat killed Burkholderia sp. strain A396 as seed treatment and foliar application) and Gaucho® (imidacloprid) (chemical check) for the P. cruciferae management at two locations (Conrad and Sweetgrass) of Montana in 2016. Biopesticide products were evaluated based on canola leaf area injury ratings and seed yield levels. Although, there was no clear trend of canola yield increase, selected biopesticide treatments were effective in maintaining low leaf area injury ratings as compared to untreated control. Entrust was able to maintain low leaf area injury ratings (8.5–14.5%) when compared to untreated control (16.0–21.4%) at both the locations. Entomopathogenic nematodes, Steinernema feltiae + Barricade® and Venerate® applied as foliar treatments maintained significantly lower feeding injury pressure at Sweetgrass (11.8%) and Conrad (13.4%) locations respectively, when compared to the untreated control. Our study results suggest that these biopesticide treatment results were comparable in efficacy to the chemical seed treatment Gaucho®. Other two biopesticide products- Aza-Direct® and Pyganic 1.4® EC treatments did not provide effective control of P. cruciferae at both the locations.Item Field efficacy of Bacillus thuringiensis galleriae strain SDS-502 for the management of alfalfa weevil and its impact on Bathyplectes spp. parasitization rate(2018-03) Shrestha, Govinda; Reddy, Gadi V. P.; Jaronski, Stefan T.Alfalfa weevil, Hypera postica Gyllenhal, is an important pest in forage alfalfa worldwide, and especially so on the Northern Plains of North America. Neither the weevil-specific fungus, Erynia phytonomi, nor the weevil's parasitoids are able to routinely suppress outbreaks as they do in the eastern U.S. A new Bacillus thuringiensis var. galleriae, having a Cry8Da coleopteran-active toxin, has been recently commercialized. We examined the efficacy of this B. thuringiensis product against the H. postica in replicated field trials in north central Montana. Because it has been suggested that efficiency of the parasitoids, Bathyplectes curculionis and Oomyzus incertus, was inversely proportional to host numbers (i.e., parasitoid efficiency increased when host population is low), we also sought to determine if a partial reduction of larval H. postica populations with a B. thuringiensis would yield to greater parasitoid efficiency, manifested as higher percent parasitism among the surviving larvae. The B. thuringiensis gave 27-40% reduction in weevil numbers at the low label rate, 55-59% for the high label rate. Mean parasitism at the two research locations varied from 5-26% and 17-36% respectively, but application of the B. thuringiensis had no significant effect on parasitism levels, i.e. parasitism was not greater in treated than in carrier control plots.Item Comparison of Thermal Performance Equations in Describing Temperature-Dependent Developmental Rates of Insects: (III) Phenological Applications(2018-11) Shi, Pei-Jian; Fan, Mei-Ling; Reddy, Gadi V. P.The developmental times of poikilotherms at different stages are significantly affected by temperature. Most mathematical models describing the temperature-dependent developmental rates of poikilotherms are built according to the experimental data at various constant temperatures. However, these models can also be applied to the developmental rates at variable temperatures. It is more meaningful to use models to predict the occurrence times of pest insects that actually represent the completion for a particular developmental stage (e.g., hatching, pupation, eclosion) under a natural thermal environment. For some developmental stages, insects might experience a period of high temperatures. In this case, skewed bell-shaped nonlinear models are more reasonable than the linear and exponential models because in the high-temperature region the developmental rate decreases with temperature increasing. We used the accumulated developmental progress method that combines three representative nonlinear models to compare the model validity in predicting the egg\'s earliest hatching date of bamboo locust in different years. We found that for the springtime phenological event the simple Arrhenius\' equation obtains the best goodness of fit. This study also provides a general R function that permits users to employ nonlinear parametric models to predict the occurrence times of insect phenology. In fact, if the investigation data cannot reflect the temperature-based phenological models proposed here, we have to consider whether the data set is reliable or whether the temperature is the crucial factor that determines the occurrence time of interest. The present study is valuable for the integrated management of pest insects because the biological or chemical control timing relies on the prediction on the occurrence time of phenological events.Item Arthropod Development's Response to Temperature: a Review and New Software for Modeling(2018-11) Mirhosseini, Mohammad A.; Fathipour, Yaghoub; Reddy, Gadi V. P.Among environmental factors, temperature has a direct and crucial influence on the key life processes of survival, development, reproduction, and movement of poikilothermic animals and hence their population dynamics. The lower and upper temperature thresholds and optimal temperature have ramifications for all major life processes, where within a specific range, a temperature change results in a proportional rise or fall of the rate of any given process. There are some basic approaches for modeling development response to temperature in arthropods. The oldest and most widely used model is a simple linear description of arthropod development in relation to temperature. The linear model forms the basis of the well-known thermal summation or degree-day (DD) approach to timing prediction. The second approach for predicting arthropod development rate is nonlinear mathematical models. However, given the fundamental role of thermal models in forecasting and integrated pest management programs, the main purpose of this paper was to facilitate the use of thermal models to estimate the developmental response of arthropods to temperature and select the appropriate model with a view to goodness-of-fit and generalizability by designing a user-friendly software. In this paper, the linear and most common nonlinear thermal models and the main criteria for selecting appropriate models are also reviewed.