High Potential for Biomass-Degrading Enzymes Revealed by Hot Spring Metagenomics

dc.contributor.authorReichart, Nicholas J.
dc.contributor.authorBowers, Robert M.
dc.contributor.authorWoyke, Tanja
dc.contributor.authorHatzenpichler, Roland
dc.date.accessioned2022-05-09T21:37:59Z
dc.date.available2022-05-09T21:37:59Z
dc.date.issued2021-04
dc.description.abstractEnzyme stability and activity at elevated temperatures are important aspects in biotechnological industries, such as the conversion of plant biomass into biofuels. In order to reduce the costs and increase the efficiency of biomass conversion, better enzymatic processing must be developed. Hot springs represent a treasure trove of underexplored microbiological and protein chemistry diversity. Herein, we conduct an exploratory study into the diversity of hot spring biomass-degrading potential. We describe the taxonomic diversity and carbohydrate active enzyme (CAZyme) coding potential in 71 publicly available metagenomic datasets from 58 globally distributed terrestrial geothermal features. Through taxonomic profiling, we detected a wide diversity of microbes unique to varying temperature and pH ranges. Biomass-degrading enzyme potential included all five classes of CAZymes and we described the presence or absence of genes encoding 19 glycosyl hydrolases hypothesized to be involved with cellulose, hemicellulose, and oligosaccharide degradation. Our results highlight hot springs as a promising system for the further discovery and development of thermostable biomass-degrading enzymes that can be applied toward generation of renewable biofuels. This study lays a foundation for future research to further investigate the functional diversity of hot spring biomass-degrading enzymes and their potential utility in biotechnological processing.en_US
dc.identifier.citationReichart, N. J., Bowers, R. M., Woyke, T., & Hatzenpichler, R. (2021). High potential for biomass-degrading enzymes revealed by hot spring metagenomics. Frontiers in microbiology, 12.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/16767
dc.language.isoen_USen_US
dc.publisherFrontiers Media SAen_US
dc.titleHigh Potential for Biomass-Degrading Enzymes Revealed by Hot Spring Metagenomicsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage13en_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.citation.volume12en_US
mus.data.thumbpage3en_US
mus.identifier.doi10.3389/fmicb.2021.668238en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.relation.universityMontana State University - Bozemanen_US

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