Volunteer Accuracy in a Benthic Macroinvertebrate Participatory Science Project

Abstract

Concerns about the accuracy of volunteer-derived aquatic macroinvertebrate identifications and the resulting influence on calculated water quality metrics can limit use of volunteer-generated data in freshwater-focused participatory science programs. To address these concerns, we assessed 357 benthic macroinvertebrate quality control (QC) samples collected by volunteers using leaf packs, kick nets, and visual assessments between 2011 and 2016 for the Environmental Quality Institute (EQI) in North Carolina, USA. We reviewed each sample for quality, and compared macroinvertebrate identifications and water quality metric scores determined by volunteers to identification and metrics determined by an entomologist. About 80% of the QC samples were identified to be of high quality, indicating proper preparation, preservation, and labeling of samples. The majority of samples that received low quality ratings were improperly or poorly preserved. We recommend use of 95% (rather than 70%) ethanol for macroinvertebrate preservation, and increased communication to volunteers during QC sampling periods to enhance their success in properly preserving samples. We observed significant (p < 0.005) linear relationships between volunteer and entomologist-derived water quality metrics including a biotic index, Taxa Richness, and percent intolerant Ephemeroptera, Plecoptera and Trichoptera (EPT) taxa. However, visual assessments – those in which no sampling equipment was used to collect aquatic macroinvertebrates – reduced the goodness of fit between volunteer and entomologist-derived biotic index scores and artificially increased the goodness of fit between volunteer and entomologist derived Taxa Richness and percent EPT scores. We recommend calculating water quality metrics based only on leaf pack and kick net samples collected by volunteers.