Physiological, elemental composition, and proteomic analyses of Escherichia coli ammonium-limited chemostat growth with comparison to iron- and glucose-limited chemostat growth.

dc.contributor.authorFolsom, James Patrick
dc.contributor.authorCarlson, Ross P.
dc.date.accessioned2016-08-10T14:07:42Z
dc.date.available2016-08-10T14:07:42Z
dc.date.issued2015-08
dc.description.abstractEscherichia coli physiological, biomass elemental composition and proteome acclimations to ammonium-limited chemostat growth were measured at four levels of nutrient scarcity controlled via chemostat dilution rate. These data were compared with published iron- and glucose-limited growth data collected from the same strain and at the same dilution rates to quantify general and nutrient-specific responses. Severe nutrient scarcity resulted in an overflow metabolism with differing organic byproduct profiles based on limiting nutrient and dilution rate. Ammonium-limited cultures secreted up to 35  % of the metabolized glucose carbon as organic byproducts with acetate representing the largest fraction; in comparison, iron-limited cultures secreted up to 70  % of the metabolized glucose carbon as lactate, and glucose-limited cultures secreted up to 4  % of the metabolized glucose carbon as formate. Biomass elemental composition differed with nutrient limitation; biomass from ammonium-limited cultures had a lower nitrogen content than biomass from either iron- or glucose-limited cultures. Proteomic analysis of central metabolism enzymes revealed that ammonium- and iron-limited cultures had a lower abundance of key tricarboxylic acid (TCA) cycle enzymes and higher abundance of key glycolysis enzymes compared with glucose-limited cultures. The overall results are largely consistent with cellular economics concepts, including metabolic tradeoff theory where the limiting nutrient is invested into essential pathways such as glycolysis instead of higher ATP-yielding, but non-essential, pathways such as the TCA cycle. The data provide a detailed insight into ecologically competitive metabolic strategies selected by evolution, templates for controlling metabolism for bioprocesses and a comprehensive dataset for validating in silico representations of metabolism.en_US
dc.description.sponsorshipZDYE LLC; National Institutes of Health (P20RR024237); National Science Foundation Integrative Graduate Education and Research Traineeship program (DGE 0654336); The Murdock Charitable Trust provided generous support for the instrumentation in the Mass Spectrometry Facility.en_US
dc.identifier.citationFolsom, James Patrick, and Ross P. Carlson. “Physiological, Biomass Elemental Composition and Proteomic Analyses of Escherichia Coli Ammonium-Limited Chemostat Growth, and Comparison with Iron- and Glucose-Limited Chemostat Growth.” Microbiology 161, no. 8 (August 1, 2015): 1659–1670. doi:10.1099/mic.0.000118.en_US
dc.identifier.issn1350-0872
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/9982
dc.titlePhysiological, elemental composition, and proteomic analyses of Escherichia coli ammonium-limited chemostat growth with comparison to iron- and glucose-limited chemostat growth.en_US
dc.typeArticleen_US
mus.citation.extentfirstpage1659en_US
mus.citation.extentlastpage1670en_US
mus.citation.issue8en_US
mus.citation.journaltitleMicrobiologyen_US
mus.citation.volume161en_US
mus.data.thumbpage8en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1099/mic.0.000118en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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