Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3

dc.contributor.authorWolfenden, Mark
dc.contributor.authorCousin, Jonathan G.
dc.contributor.authorNangia-Makker, Pratima
dc.contributor.authorRaz, Avraham
dc.contributor.authorCloninger, Mary
dc.date.accessioned2015-10-16T21:20:53Z
dc.date.available2015-10-16T21:20:53Z
dc.date.issued2015-04
dc.description.abstractMultivalent protein-carbohydrate interactions that are mediated by sugar-binding proteins, i.e., lectins, have been implicated in a myriad of intercellular recognition processes associated with tumor progression such as galectin-mediated cancer cellular migration/metastatic processes. Here, using a modified ELISA, we show that glycodendrimers bearing mixtures of galactosides, lactosides, and N-acetylgalactosaminosides, galectin-3 ligands, multivalently affect galectin-3 functions. We further demonstrate that lactose functionalized glycodendrimers multivalently bind a different member of the galectin family, i.e., galectin-1. In a modified ELISA, galectin-3 recruitment by glycodendrimers was shown to directly depend on the ratio of low to high affinity ligands on the dendrimers, with lactose-functionalized dendrimers having the highest activity and also binding well to galectin-1. The results depicted here indicate that synthetic multivalent systems and upfront assay formats will improve the understanding of the multivalent function of galectins during multivalent protein carbohydrate recognition/interaction.en_US
dc.description.sponsorshipNIH GM62444en_US
dc.identifier.citationWolfenden, Mark, Jonathan Cousin, Pratima Nangia-Makker, Avraham Raz, and Mary Cloninger. "Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3." Molecules 20, no. 4 (April 20, 2015): 7059-7096. DOI:https://dx.doi.org/10.3390/molecules20047059.en_US
dc.identifier.issn1420-3049
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/9328
dc.rightsCC BY 4.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleGlycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3en_US
dc.typeArticleen_US
mus.citation.extentfirstpage7059en_US
mus.citation.extentlastpage7096en_US
mus.citation.issue4en_US
mus.citation.journaltitleMoleculesen_US
mus.citation.volume20en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryChemical & Material Science
mus.identifier.doi10.3390/molecules20047059en_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.universityMontana State University - Bozemanen_US

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