Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane

dc.contributor.authorMcKay, Luke J.
dc.contributor.authorSmith, Heidi J.
dc.contributor.authorBarnhart, Elliott P.
dc.contributor.authorSchweitzer, Hannah D.
dc.contributor.authorMalmstrom, Rex R.
dc.contributor.authorGoudeau, Danielle
dc.contributor.authorFields, Matthew W.
dc.date.accessioned2022-04-28T22:25:49Z
dc.date.available2022-04-28T22:25:49Z
dc.date.issued2021-10
dc.description.abstractMicrobial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarbons. From the active fraction, high-quality metagenome-assembled genomes (MAGs) were recovered for the acetoclastic methanogen, Methanothrix paradoxum, and a novel member of the Chlorobi with the potential to generate acetate via the Pta-Ack pathway. Members of the Bacteroides and Geobacter also encoded Pta-Ack and together, all four populations had the putative ability to degrade ethylbenzene, phenylphosphate, phenylethanol, toluene, xylene, and phenol. Metabolic reconstructions, gene analyses, and environmental parameters also indicated that redox fluctuations likely promote facultative energy metabolisms in the coal seam. The active "Chlorobi PRB" MAG encoded enzymes for fermentation, nitrate reduction, and multiple oxygenases with varying binding affinities for oxygen. "M. paradoxum PRB" encoded an extradiol dioxygenase for aerobic phenylacetate degradation, which was also present in previously published Methanothrix genomes. These observations outline underlying processes for bio-methane from subbituminous coal by translationally active populations and demonstrate activity-based metagenomics as a powerful strategy in next generation physiology to understand ecologically relevant microbial populations.en_US
dc.identifier.citationMcKay, L., Smith, H., Barnhart, E., Schweitzer, H., Malmstrom, R., Goudeau, D., & Fields, M. (2021). Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane. The ISME journal, 16(4), 915-926. http://dx.doi.org/10.1038/s41396-021-01139-xen_US
dc.identifier.issn1751-7362
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/16753
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.titleActivity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methaneen_US
dc.typeArticleen_US
mus.citation.extentfirstpage915en_US
mus.citation.extentlastpage926en_US
mus.citation.issue4en_US
mus.citation.journaltitleThe ISME journalen_US
mus.citation.volume16en_US
mus.data.thumbpage19en_US
mus.identifier.doi10.1038/s41396-021-01139-xen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.relation.universityMontana State University - Bozemanen_US

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