Viable real-time PCR in environmental samples: Can all data be interpreted directly?

dc.contributor.authorFittipaldi, M.
dc.contributor.authorCodony, F.
dc.contributor.authorAdrados, B.
dc.contributor.authorCamper, Anne K.
dc.contributor.authorMorato, J.
dc.date.accessioned2017-02-07T16:36:29Z
dc.date.available2017-02-07T16:36:29Z
dc.date.issued2011-01
dc.description.abstractSelective nucleic acid intercalating dyes—ethidium monoazide (EMA) and propidium monoazide (PMA)—represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limitations were found, especially in environmental samples. The aim of this work was to show that in the application of viable real-time PCR, there may be significant biases and to propose a strategy for overcoming some of these problems. We present an approach based on the combination of three real-time PCR amplifications for each sample that should provide an improved estimation of the number of viable cells. This approach could be useful especially when it is difficult to determine a priori how to optimize methods using PMA or EMA. Although further studies are required to improve viable real-time PCR methods, the concept as outlined here presents an interesting future research direction.en_US
dc.identifier.citationFittipaldi M, Codony F, Adrados B, Camper AK, Morató J, "Viable real-time PCR in environmental samples: Can all data be interpreted directly?," Microbial Ecology, January 2011 61(1):7–12en_US
dc.identifier.issn0095-3628
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12573
dc.titleViable real-time PCR in environmental samples: Can all data be interpreted directly?en_US
dc.typeArticleen_US
mus.citation.extentfirstpage7en_US
mus.citation.extentlastpage12en_US
mus.citation.issue1en_US
mus.citation.journaltitleMicrobial Ecologyen_US
mus.citation.volume61en_US
mus.data.thumbpage5en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s00248-010-9719-1en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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