A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa

dc.contributor.authorAryal, Niranjan
dc.contributor.authorLu, Chaofu
dc.date.accessioned2019-02-08T19:03:31Z
dc.date.available2019-02-08T19:03:31Z
dc.date.issued2018-11
dc.description.abstractThere have been strong interests in producing unusual fatty acids in oilseed crops to provide renewable industrial feedstock. Results are so far largely disappointing since much lower amounts of such fatty acids accumulate in genetically engineered seeds than in their original natural sources. It has been suggested that the flux of unusual fatty acids through phosphatidylcholine (PC) represents a major bottleneck for high accumulation of such fatty acids in triacylglycerol (TAG). We show here that a phospholipase C-like protein (RcPLCL1) from castor bean, which accumulates nearly 90% of the hydroxylated ricinoleic acid in its seed TAG, increases the amount of hydroxy fatty acids (HFAs) when co-expresses with the fatty acid hydroxylase (RcFAH12) in transgenic seed of Camelina sativa. RcPLCL1 shows hydrolyzing activities on both PC and phosphatidylinositol substrates in our in vitro assay conditions. The PC-PLC activity of the RcPLCL1 may have increased the efficiency of HFA-PC to diacylglycerol conversion, which explains our observation of increased HFA contents in TAG concomitant with decreased HFA in the membrane lipid PC during seed development. Consequently, this may also alleviate the potential detrimental effect of HFA on germination of the engineered camelina seeds. Our results provide new knowledge that will help design effective strategies to engineer high levels of HFAs in transgenic oilseeds.en_US
dc.description.sponsorshipNSF (DBI-1339358 ); USDA (2015-67013-22815); Hatch Project (1015638)en_US
dc.identifier.citationAryal, Niranjan, and Chaofu Lu. "A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa." FRONTIERS IN PLANT SCIENCE 9 (November 2018). DOI:10.3389/fpls.2018.01576.en_US
dc.identifier.issn1664-462X
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/15235
dc.language.isoenen_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleA Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativaen_US
dc.typeArticleen_US
mus.citation.journaltitleFrontiers in Plant Scienceen_US
mus.citation.volume9en_US
mus.contributor.orcidLu, Chaofu|0000-0001-6901-7490en_US
mus.data.thumbpage6en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.3389/fpls.2018.01576en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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