Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgels

dc.contributor.authorFredrikson, Jacob P.
dc.contributor.authorBrahmachary, Priyanka P.
dc.contributor.authorErdoğan, Ayten E.
dc.contributor.authorArchambault, Zachary K.
dc.contributor.authorWilking, James N.
dc.contributor.authorJune, Ronald K.
dc.contributor.authorChang, Connie B.
dc.date.accessioned2022-09-30T22:00:02Z
dc.date.available2022-09-30T22:00:02Z
dc.date.issued2022-03
dc.description.abstractArticular cartilage is comprised of two main components, the extracellular matrix (ECM) and the pericellular matrix (PCM). The PCM helps to protect chondrocytes in the cartilage from mechanical loads, but in patients with osteoarthritis, the PCM is weakened, resulting in increased chondrocyte stress. As chondrocytes are responsible for matrix synthesis and maintenance, it is important to understand how mechanical loads affect the cellular responses of chondrocytes. Many studies have examined chondrocyte responses to in vitro mechanical loading by embedding chondrocytes in 3-D hydrogels. However, these experiments are mostly performed in the absence of PCM, which may obscure important responses to mechanotransduction. Here, drop-based microfluidics is used to culture single chondrocytes in alginate microgels for cell-directed PCM synthesis that closely mimics the in vivo microenvironment. Chondrocytes formed PCM over 10 days in these single-cell 3-D microenvironments. Mechanotransduction studies were performed, in which single-cell microgels mimicking the cartilage PCM were embedded in high-stiffness agarose. After physiological dynamic compression in a custom-built bioreactor, microgels exhibited distinct metabolomic profiles from both uncompressed and monolayer controls. These results demonstrate the potential of single cell encapsulation in alginate microgels to advance cartilage tissue engineering and basic chondrocyte mechanobiology.en_US
dc.identifier.issn2073-4409
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/17269
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.rightscc-byen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectchondrocytesen_US
dc.subjectmicrogelsen_US
dc.subjectmicrofluidicsen_US
dc.subjectmechanotransductionen_US
dc.subjectosteoarthritisen_US
dc.subjectpericellular matrixen_US
dc.subjectmetabolomicsen_US
dc.titleMetabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgelsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage18en_US
mus.citation.issue5en_US
mus.citation.journaltitleCellsen_US
mus.citation.volume11en_US
mus.data.thumbpage7en_US
mus.identifier.doi10.3390/cells11050900en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
fredrikson-microgels-2022.pdf
Size:
2.42 MB
Format:
Adobe Portable Document Format
Description:
alginate microgels
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
826 B
Format:
Item-specific license agreed upon to submission
Description:
Copyright (c) 2002-2022, LYRASIS. All rights reserved.