Scholarly Work - Animal & Range Sciences
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8931
Browse
2 results
Search Results
Item Application of Feeding Behaviour to Predict Morbidity of Newly Received Calves in a Commercial Feedlot.(2001-09) Quimby, W. F.; Sowell, Bok F.; Bowman, J. G. P; Branine, M. E.; Hubbert, M. E.; Sherwood, H. W.The objective of this study was to use feeding behavior of newly received steers (average initial weight 191 kg) to detect morbidity in animals in a commercial feedlot. Two separate 32 d feeding trials were conducted in Wellton, Arizona, in July and November 1996. Radio frequency technology was used to record the total time spent within 50 cm of the feedbunk (animal presence every 5.25 s times 5.25 s) in 3 h intervals from 0600 to 2400 on a daily basis for 103 and 122 male calves in trial 1 and 2, respectively. Statistical procedures based on the cumulative sums (CUSUM) of the 3 h feeding intervals were used to detect morbid animals, compared with detection of animals deemed morbid by experienced pen riders. In trial 1, the CUSUM procedure detected animal morbidity 4.5 d earlier (P < 0.001) than the feedlot personnel. In trial 2, the CUSUM procedure detected animal morbidity 3.7 d earlier (P < 0.001) than feedlot pen riders. Overall accuracy, positive predictive value and sensitivity of the CUSUM prediction method were 87, 91, and 90%, respectively. Combined trial data suggest that feeding behavior during the first 30 d cattle are in a receiving pen, as collected with radio frequency technology and analyzed with CUSUM charts, may be used to detect animal morbidity approximately 4.1 d earlier (P < 0.001) than conventional methods typically employed in commercial feedlots.Item TmpL, a transmembrane protein required for intracellular redox homeostasis and virulence in a plant and an animal fungal pathogen(2009-11) Kim, Kwang-Hyung; Willger, Sven D.; Park, Sang-Wook; Puttikamonkul, Srisombat; Grahl, Nora; Cho, Yangrae; Mukhopadhyay, Biswarup; Cramer, Robert A.; Lawrence, Christopher B.The regulation of intracellular levels of reactive oxygen species (ROS) is critical for developmental differentiation and virulence of many pathogenic fungi. In this report we demonstrate that a novel transmembrane protein, TmpL, is necessary for regulation of intracellular ROS levels and tolerance to external ROS, and is required for infection of plants by the necrotroph Alternaria brassicicola and for infection of mammals by the human pathogen Aspergillus fumigatus. In both fungi, tmpL encodes a predicted hybrid membrane protein containing an AMP-binding domain, six putative transmembrane domains, and an experimentally-validated FAD/NAD(P)-binding domain. Localization and gene expression analyses in A. brassicicola indicated that TmpL is associated with the Woronin body, a specialized peroxisome, and strongly expressed during conidiation and initial invasive growth in planta. A. brassicicola and A. fumigatus DtmpL strains exhibited abnormal conidiogenesis, accelerated aging, enhanced oxidative burst during conidiation, and hypersensitivity to oxidative stress when compared to wild-type or reconstituted strains. Moreover, A. brassicicola DtmpL strains, although capable of initial penetration, exhibited dramatically reduced invasive growth on Brassicas and Arabidopsis. Similarly, an A. fumigatus DtmpL mutant was dramatically less virulent than the wild-type and reconstituted strains in a murine model of invasive aspergillosis. Constitutive expression of the A. brassicicola yap1 ortholog in an A. brassicicola DtmpL strain resulted in high expression levels of genes associated with oxidative stress tolerance. Overexpression of yap1 in the DtmpL background complemented the majority of observed developmental phenotypic changes and partially restored virulence on plants. Yap1-GFP fusion strains utilizing the native yap1 promoter exhibited constitutive nuclear localization in the A. brassicicola DtmpL background. Collectively, we have discovered a novel protein involved in the virulence of both plant and animal fungal pathogens. Our results strongly suggest that dysregulation of oxidative stress homeostasis in the absence of TmpL is the underpinning cause of the developmental and virulence defects observed in these studies.