Scholarly Work - Land Resources & Environmental Sciences
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8680
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Item Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America(Elsevier BV, 2023-08) Toševski, Ivo; Sing, Sharlene E.; Caldara, Roberto; Weaver, David K.; Jović, Jelena; Krstić, Oliver; Hinz, Hariet L.A seed-feeding weevil introduced to North America (NA) as a biological control agent of the invasive toadflax Linaria dalmatica (L.) Mill., identified then as Gymnetron antirrhini “Dalmatian host race” and subsequently confirmed as established, was revealed through our study to be a separate species, i.e., Rhinusa dieckmanni (Behne) (Coleoptera: Curculionidae). This weevil species was presumed to be endemic in its native range, with a distribution restricted to Mount Rila in southwestern Bulgaria. We conducted a comprehensive study of seed-feeding weevils associated with L. dalmatica, L. dalmatica ssp. macedonica (Griseb.) D.A. Sutton, L. genistifolia (L.) Mill., and L. grandiflora Desf. across a broad geographic area of their native range. Those results revealed that all four host plants were used by R. dieckmanni and thus the native geographic range of the species is wider than expected, encompassing the Balkans and the Anatolian Plateau. Our observations suggest that phenotypes of this weevil are highly variable and dependent on the seed capsule size of the Linaria host population. The haplotype network based on mitochondrial COII, 16S genes, and nuclear EF 1-α gene genealogy confirmed the conspecific nature of geographically distant weevil populations, that is, R. dieckmanni phenotypes utilizing L. genistifolia, L. dalmatica, and L. grandiflora for larval development. Specimens collected from L. dalmatica in the northwestern USA shared the same haplotypes as samples from L. dalmatica ssp. macedonica in southwestern North Macedonia, supporting the known introduction history of the North American population. Females from these populations have relatively short rostrums, which may limit their reproductive success on North American invasive L. dalmatica with larger seed capsules.Item Cowpea extrafloral nectar has potential to provide ecosystem services lost in agricultural intensification and support native parasitoids that suppress the wheat stem sawfly(Oxford Academic, 2023-05) Cavallini, Laissa; Peterson, Robert K. D.; Weaver, David K.The native parasitoids Bracon cephi (Gahan) and B. lissogaster Muesebeck (Hymenoptera: Braconidae) reduce populations of Cephus cinctus Norton (Hymenoptera: Cephidae), a native grassland species, and major wheat pest on the Northern Great Plains of North America. Non-host feeding adults of these braconids increase longevity, egg load, and egg volume when provisioned carbohydrate-rich diets. Nutrition from nectar can enhance the success of natural enemies in pest management programs. Cowpea, Vigna unguiculata (L.) Walpers, is a potential cover-crop that could add resilient features to the landscape and has extrafloral nectaries (EFN), easy-access nectar sources for beneficial insects. If more cowpea was grown on the Northern Great Plains, would B. cephi and B. lissogaster benefit from foraging on putatively beneficial EFN? We investigated cowpea inflorescence stalk extrafloral nectars (IS-EFN) and leaf stipel extrafloral nectars (LS-EFN) as potential food sources for these parasitoids. Females were caged on EFN sources on living cowpea plants to assess longevity. Egg load and volume were measured at 2, 5, and 10 days after placement. Bracon cephi survived 10 days on water, 38 days on IS-EFN; B. lissogaster 6 days on water, 28 days on IS-EFN. Bracon lissogaster maintained a constant egg load and volume across treatments while B. cephi produced 2.1-fold more eggs that were 1.6-fold larger on IS-EFN. Y-tube olfactometry indicated adult females were attracted to airstreams containing cowpea volatiles. These results demonstrate that non-native, warm-season cowpea benefits these native parasitoids and may improve conservation biocontrol of C. cinctus.Item Using Stable Isotopes to Determine Natal Origin and Feeding Habits of the Army Cutworm Moth, Euxoa auxiliaris (Lepidoptera: Noctuidae)(Oxford University Press, 2023-02) Dittemore, Clare M.; Tyers, Daniel B.; Weaver, David K.; Nunlist, Erika A.; Sowell, Bok F.; Peterson, Erik; Peterson, Robert K. D.The army cutworm, Euxoa auxiliaris (Grote), is a migratory noctuid that is both an agricultural pest and an important late-season food source for grizzly bears, Ursus arctos horribilis (Linnaeus, Carnivora: Ursidae), within the Greater Yellowstone Ecosystem. Beyond the confirmation of the moths’ seasonal, elevational migration in the mid-1900s, little else has been documented about their migratory patterns. To address this missing ecological component, we examined (1) migratory routes during their spring and fall migratory periods throughout their natal range, the Great Plains, and (2) natal origin at two of their summering ranges using stable hydrogen (δ2H) analyses of wings from samples collected within the areas of interest. Stable carbon (δ13C) and stable nitrogen (δ15N) analyses of wings were used to evaluate larval feeding habits of the migrants and agricultural intensity of natal origin sites, respectively. Results suggest that, rather than migrating exclusively east to west, army cutworm moths are also migrating north to south during their spring migration. Moths did not exhibit natal origin site fidelity when returning to the Great Plains. Migrants collected from the Absaroka Range had the highest probability of natal origin in Alberta, British Columbia, Saskatchewan, the most southern region of the Northwest Territories, and second highest probability of origin in Montana, Wyoming, and Idaho. Migrants collected in the Lewis Range had the highest probability of origin in the same provinces of Canada. Results suggest that migrants of the Absaroka Range fed exclusively on C3 plants as larvae and rarely fed in heavily fertilized agroecosystems.Item Dietary sugars and amino acids increase longevity and enhance reproductive parameters of Bracon cephi and B. lissogaster , two parasitoids that specialise on wheat stem sawfly(Wiley, 2023) Cavallini, Laissa; Peterson, Robert K. D.; Weaver, David K.The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a major pest of wheat grown on the Northern Great Plains and Canadian Prairies of North America, causing economic losses of as much as $350 million annually. Two species that parasitize wheat stem sawfly larvae in wheat stems are Bracon cephi (Gahan) and B. lissogaster Muesebeck (Hymenoptera: Braconidae). Carbohydrate-rich diets increase adult parasitoid longevity and reproductive parameters, enhancing their success as natural enemies. In previous studies, these species had increased longevity, egg load, and volume when fed sucrose solutions, encouraging further research on their nutritional needs. Therefore, we conducted experiments with artificial diets using adult females fed sucrose, glucose, and fructose solutions. Females were also fed these sugars in combination with a mixture of amino acids. We assessed individuals daily for longevity. Both species benefited from diets containing carbohydrates, with an increase in longevity from an average of 9 to 51 days for B. cephi, and from 6 to 34 days for B. lissogaster. Additional experiments assessed egg load and volume after 2, 5, and 10 days of cumulative feeding in B. cephi. These females produced 1.4-fold more eggs when fed amino acids, 2.5-fold more with sugar, and 2.7-fold more when fed sugar with amino acids. They had a 1.3-fold increase in egg volume when fed amino acids, 1.9-fold with sugar, and 2.1-fold when fed sugar with amino acids. Our study reveals the nutritional requirements of these braconid parasitoids and the benefits of nutritional sources when implementing conservation biological control strategies.Item Cropping systems alter plant volatile emissions in the field through soil legacy effects(Cambridge University Press, 2022-06) Malone, Shealyn C.; Menalled, Fabian D.; Weaver, David K.; Seipel, Tim F.; Hofland, Megan L.; Runyon, Justin B.; Bourgault, Maryse; Boss, Darrin L.; Trowbridge, Amy M.Crops emit a variety of volatile organic compounds (VOCs) that serve as attractants or repellents for pests and their natural enemies. Crop rotations, off-farm chemical inputs, and mechanical and cultural tactics – collectively called cropping systems – alter soil nutrients, moisture content, and microbial communities, all of which have the potential to alter crop VOC emissions. Soil legacy effects of diversified cropping systems have been shown to enhance crop VOC emissions in greenhouse studies, but how they influence emissions under field conditions remains virtually unknown. To determine the effect of cropping systems on plant VOC emissions in the field, air samples were collected from the headspace of wheat (Triticum aestivum L. Judee) grown in simplified wheat-fallow rotations or diversified wheat-cover crop rotations where cover crops were terminated by grazing cattle. Across two growing seasons, wheat grown in rotation with fallow emitted greater amounts of Z-3-hexenyl acetate and β-ocimene, key attractants for wheat stem sawfly (Cephus cinctus Norton), a major pest of wheat. While overall VOC blends were relatively similar among cropping system during the first growing season, emissions varied substantially in the second year of this study where wheat grown in rotation with cover crops emitted substantially greater quantities of volatile compounds characteristic of abiotic stress. Below-average precipitation in the second growing season, in addition to reduced soil water content in cover crop rotations, suggests that cropping system effects on wheat VOCs may have been driven primarily by water availability, a major factor limiting crop growth in dryland agriculture. While the specific mechanisms driving changes in VOC emissions were not explicitly tested, this work shows that agricultural practices applied in one growing season can differentially influence crop VOC emissions in the next through soil legacy effects, illustrating additional avenues through which cropping systems may be leveraged to enhance pest management.Item Stability analysis of stem solidness, grain yield, and grain protein concentration in spring wheat(Canadian Science Publishing, 2021-08) Subedi, Maya; Cárcamo, Héctor A.; Knodel, Janet J.; Weaver, David K.; Cuthbert, Richard D.; Pozniak, Curtis J.; Nilsen, Kirby T.; Beres, Brian L.The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a major pest of wheat (Triticum aestivum L.) in the northern Great Plains, where it is a constant threat in Montana and is resurging in Alberta, Saskatchewan, and North Dakota. Adoption of solid-stemmed cultivars is an important management tool for wheat growers; however, the inconsistent pith expression first noted with the release of ‘Rescue’ has been repeatedly observed in modern cultivars such as ‘Lillian’ in Canada. Given the extensive hectares planted to solid-stemmed wheat cultivars during an outbreak, the identification of cultivars that display stable stem solidness, grain yield, and grain protein concentration across a wide range of environments where stem sawfly infestations occur is desirable. We assessed spring wheat plant responses in eight solid-stemmed and two hollow-stemmed genotypes grown across diverse environments using multiple statistical models. Study sites included southern Alberta and Saskatchewan, Montana, and North Dakota. Most models agreed that the genotypes ‘Choteau’, ‘BW925’, and ‘Mott’ consistently displayed high and stable stem solidness concomitant with high grain yield. ‘Choteau’ and ‘BW925’ also consistently met or exceeded the desired threshold of a 3.75/5 pith rating (averaged from the lower four stem internodes) for optimum resistance, whereas ‘Mott’ developed optimal pith at a specific (early) phenological stage when resistance to wheat stem sawfly infestation is critical. Exploring the stability of stem solidness identified ideal genotypes that would enhance germplasm development efforts, which exemplifies how this approach can facilitate the selection, production, and adoption of solid-stemmed wheat cultivars in regions prone to wheat stemItem Impacts of Dryland Cropping Systems on Ground Beetle Communities (Coleoptera: Carabidae) in the Northern Great Plains(Oxford University Press, 2021-01) DuPre, Mary E.; Weaver, David K.; Seipel, Tim F.; Menalled, Fabian D.Ground beetles are natural predators of insect pests and small seeds in agroecosystems. In semiarid cropping systems of the Northern Great Plains, there is a lack of knowledge to how ground beetles are affected by diversified cover crop rotations. In a 2-yr study (2018 and 2019), our experiment was a restricted-randomization strip-plot design, comprising summer fallow, an early-season cover crop mixture (five species), and a mid-season cover crop mixture (seven species), with three cover crop termination methods (i.e., herbicide, grazing, and haying). Using pitfall traps, we sampled ground beetles in five 48-h intervals throughout the growing season (n = 135 per year) using growing degree day (GDD) accumulations to better understand changes to ground beetle communities. Data analysis included the use of linear mixed-effects models, perMANOVA, and non-metric multidimensional scaling ordinations. We did not observe differences among cover crop termination methods; however, activity density in the early-season cover crop mixture decreased and in summer fallow increased throughout the growing season, whereas the mid-season cover crop mixture peaked in the middle of the summer. Ground beetle richness and evenness showed a nonlinear tendency, peaking in the middle of the growing season, with marginal differences between cover crops or fallow after the termination events. Also, differences in ground beetle composition were greatest in the early- and mid-season cover crop mixtures earlier in the growing season. Our study supports the use of cover crop mixtures to enhance ground beetle communities, with potential implications for pest management in dryland cropping systems.Item Plant Volatiles and Oviposition Behavior in the Selection of Barley Cultivars by Wheat Stem Sawfly (Hymenoptera: Cephidae)(Oxford University Press, 2021-08) Achhami, Buddhi B.; Reddy, Gadi V. P.; Hofland, Megan L.; Sherman, Jamie D.; Peterson, Robert K. D.; Weaver, David K.Wheat stem sawfly, [Cephus cinctus (Hymenoptera: Cephidae)], females display complex behaviors for host selection and oviposition. Susceptible hollow stem wheat (Triticum aestivum L.) cultivars release a greater amount of attractive compound, (Z)-3-hexenyl acetate and receive a greater number of eggs compared to resistant solid stem wheat cultivars. However, barley (Hordeum vulgare L.) is becoming a more common host for C. cinctus in Montana. Therefore, how do host selection and oviposition behaviors on barley cultivars compare to what happens when encountering wheat cultivars? To answer this question, we carried out greenhouse experiments using two barley cultivars: ‘Hockett’ and ‘Craft’. Between these cultivars at Zadoks stages 34 and 49, we compared host selection decisions using a Y-tube olfactometer, compared oviposition behaviors on stems, and counted the number of eggs inside individual stems. In Y-tube bioassays, we found a greater number of C. cinctus females were attracted to the airstream passing over ‘Hockett’ than ‘Craft’ barley cultivars. Although the frequencies of oviposition behaviors were similar between these cultivars, the number of eggs was greater in ‘Hockett’. Volatile profiles indicated that the amount of linalool was greater in the airstream from ‘Craft’ than in ‘Hockett’ at Zadoks 34 while the amount of (Z)-3-hexenyl acetate was greater in airstream from ‘Hockett’ at both Zadoks 34 and 49. These results suggest that volatiles of barley plants influenced host selection behavior of ovipositing C. cinctus females, while other discriminating behaviors do not differ between cultivars.Item An Herbivore-Induced Plant Volatile From Saltcedar (Tamarix spp.) Is Repellent to Diorhabda carinulata (Coleoptera: Chrysomelidae)(2020-10) Gaffke, Alexander M.; Sing, Sharlene E.; Millar, Jocelyn G.; Dudley, Tom L.; Bean, Daniel W.; Peterson, Robert K. D.; Weaver, David K.The leaf beetle Diorhabda carinulata Desbrochers (Coleoptera: Chrysomelidae) was introduced into the United States in 1999 for classical biological control of the exotic woody invader saltcedar (Tamarix spp. L. [Caryophyllales: Tamaricaceae]). The recent southern expansion of the range of D. carinulata in the United States has precipitated conflict between proponents of biological control of Tamarix and those with concerns over habitat conservation for avian species. Several semiochemicals that mediate aggregations by this species have been reported, but no repellent compounds have been recorded thus far. We now report a repellent compound, 4-oxo-(E)-2-hexenal, induced by adult D. carinulata feeding on saltcedar foliage. Collection of headspace volatiles, gas chromatography mass spectrometry, and electroantennographic analyses identified 4-oxo-(E)-2-hexenal as an insect-induced compound that is antennally active. Behavioral and exposure assays were conducted to test for repellency and toxicity in adults and larvae. Headspace volatiles were also collected from adult males exposed to 4-oxo-(E)-2-hexenal to determine the impact exposure might have on the emission of the aggregation pheromone. 4-Oxo-(E)-2-hexenal elicited electrophysiological responses in adults of both sexes. Behavioral responses indicated repellency across multiple doses for reproductive D. carinulata adults but not in nonreproductive adults. Exposure assays indicated altered behaviors in first instar larvae and adults, but not in third instar larvae. Collection of headspace volatiles indicated that exposure to 4-oxo-(E)-2-hexenal did not alter emission of the D. carinulata aggregation pheromone by adult males. The continued development and field deployment of this repellent compound may provide a new tool for the management of D. carinulata.Item Antixenosis, Antibiosis, and Potential Yield Compensatory Response in Barley Cultivars Exposed to Wheat Stem Sawfly (Hymenoptera: Cephidae) under Field Conditions(2020-08) Achhami, Buddhi B.; Reddy, Gadi V. P.; Sherman, Jamie D.; Peterson, Robert K. D.; Weaver, David K.Wheat stem sawfly, Cephus cinctus Norton, is an economically serious pest of cereals grown in North America. Barley cultivars were previously planted as resistant crops in rotations to manage C. cinctus, but due to increasing levels of injury to this crop, this is no longer a valid management tactic in Montana. Therefore, we aimed to understand antixenosis (behavioral preference), antibiosis (mortality), and potential yield compensation (increased productivity in response to stem injuries) in barley exposed to C. cinctus. We examined these traits in eight barley cultivars. Antixenosis was assessed by counting number of eggs per stem and antibiosis was assessed by counting infested stems, dead larvae, and stems cut by mature larvae. Potential yield compensation was evaluated by comparing grain yield from three categories of stem infestation: 1) uninfested, 2) infested with dead larva, and 3) infested cut by mature larva at crop maturity. We found the greatest number of eggs per infested stem (1.80 ± 0.04), the highest proportion of infested stems (0.63 ± 0.01), and the highest proportion of cut stems (0.33 ± 0.01) in ‘Hockett’. Seven out of eight cultivars had greater grain weight for infested stems than for uninfested stems. These cultivars may have compensatory responses to larval feeding injury. Overall, these barley cultivars contain varying levels of antixenosis, antibiosis, and differing levels of yield compensation. Our results provide foundational knowledge on barley traits that will provide a framework to further develop C. cinctus resistant or tolerant barley cultivars.
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