Scholarly Work - Plant Sciences & Plant Pathology
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8870
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Item Identification and Characterization of Bacteria-Derived Antibiotics for the Biological Control of Pea Aphanomyces Root Rot(MDPI AG, 2022-08) Lai, Xiao; Niroula, Dhirendra; Burrows, Mary; Wu, Xiaogang; Yan, QingAntibiosis has been proposed to contribute to the beneficial bacteria-mediated biocontrol against pea Aphanomyces root rot caused by the oomycete pathogen Aphanomyces euteiches. However, the antibiotics required for disease suppression remain unknown. In this study, we found that the wild type strains of Pseudomonas protegens Pf-5 and Pseudomonas fluorescens 2P24, but not their mutants that lack 2,4-diacetylphloroglucinol, strongly inhibited A. euteiches on culture plates. Purified 2,4-diacetylphloroglucinol compound caused extensive hyphal branching and stunted hyphal growth of A. euteiches. Using a GFP-based transcriptional reporter assay, we found that expression of the 2,4-diacetylphloroglucinol biosynthesis gene phlAPf-5 is activated by germinating pea seeds. The 2,4-diacetylphloroglucinol producing Pf-5 derivative, but not its 2,4-diacetylphloroglucinol non-producing mutant, reduced disease severity caused by A. euteiches on pea plants in greenhouse conditions. This is the first report that 2,4-diacetylphloroglucinol produced by strains of Pseudomonas species plays an important role in the biocontrol of pea Aphanomyces root rot.Item Perceptions, Experiences, and Priorities Supporting Agro-ecosystem Management Decisions Differ Among Agricultural Producers, Consultants, and Researchers(2018-11) McKenzie, Sean C.; Parkinson, Hilary A.; Mangold, Jane M.; Burrows, Mary; Ahmed, Selena; Menalled, Fabian D.The sustainability of agriculture depends as much on the natural resources required for production as it does on the stakeholders that manage those resources. It is thus essential to understand the variables that influence the decision-making process of agricultural stakeholders to design educational programs, interventions, and policies geared towards their specific needs, a required step to enhance agricultural sustainability. This study employed a survey of multiple-choice and open-ended questions to examine the perceptions, experiences, and priorities that influence management decisions of agricultural stakeholders across Montana, United States. A total of 272 respondents completed a survey, representing five distinct agricultural stakeholder groups: 103 (34.9%) conventional producers, 78 (28.7%) consultants, 37 (13.6%) researchers, 33 (12.1%) organic grain producers, and 21 (7.7%) organic vegetable producers. The results revealed that, while stakeholder groups have distinct perceptions, experiences, and priorities, there were similarities across groups (pseudo-F = 22.92, p = 0.001). Specifically, organic vegetable and organic small-grain producers showed similar responses that were, in turn, divergent from those of conventional producers, researchers, and crop consultants. Conventional small-grain producers and researchers showed overlapping response patterns, while crop consultants formed an isolated group. Six clusters resulting from the classification of the multiple-choice response dataset associated with specific agricultural professions (χ2 = 549.72, p = 0.001). The classification of open-ended questions that assessed agronomic challenges and research needs resulted in six distinctive clusters, with specific associations between clusters and agricultural stakeholder groups (χ2 = 164.41, p = 0.001). These results reinforce the need for agricultural education and programs that address unique and shared experiences, priorities, and concerns of multiple stakeholder groups. This study endorses the call for a paradigm shift from the traditional top-down agricultural extension model to one that accounts for participants’ socio-ecological contexts to facilitate the adoption of sustainable agricultural systems that support environmental and human wellbeing.