Scholarly Work - Plant Sciences & Plant Pathology
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8870
Browse
3 results
Search Results
Item Functionalized para-substituted benzenes as 1,8-cineole production modulators in an endophytic Nodulisporium species(2014-08) Nigg, Jered; Strobel, Gary A.; Knighton, W. Berk; Hilmer, Jonathan K.; Geary, Brad; Ul-Hassan, Syed Riyaz; Harper, James K.; Valenti, Domenic J.; Wang, YueminA Nodulisporium species (designated Ti-13) was isolated as an endophyte from Cassia fistula. The fungus produces a spectrum of volatile organic compounds (VOCs) that includes ethanol, acetaldehyde and 1,8-cineole as major components. Initial observations of the fungal isolate suggested that reversible attenuation of the organism via removal from the host and successive transfers in pure culture resulted in a 50 % decrease in cineole production unrelated to an overall alteration in fungal growth. A compound (CPM1) was obtained from Betula pendula (silver birch) that increases the production of 1,8-cineole by an attenuated Ti-13 strain to its original level, as measured by a novel bioassay method employing a 1,8-cineole-sensitive fungus (Sclerotinia sclerotiorum). The host plant produces similar compounds possessing this activity. Bioactivity assays with structurally similar compounds such as ferulic acid and gallic acid suggested that the CPM1 does not act as a simple precursor to the biosynthesis of 1,8-cineole. NMR spectroscopy and HPLC-ES-MS indicated that the CPM1 is a para-substituted benzene with alkyl and carboxyl substituents. The VOCs of Ti-13, especially 1,8-cineole, have potential applications in the industrial, fuel and medical fields.Item 3-Carbamoylquinoxalin-1-ium chloride(2011-12) Harper, James K.; Strobel, Gary A.; Arif, Atta M.The title compound, C9H8N3O+·Cl-, was isolated from a liquid culture of streptomyces sp. In the cation, the ring system makes a dihedral angle of 0.2 (2)° with the amide group. The protonation creating the cation occurs at ome of the N atoms in the quinoxaline ring system. In the crystal, the ions are linked through N-H...O and N-H...Cl hydrogen bonds, forming a two-dimensional network parallel to (10\overline{3}).Item (6 S *)-6-[(1 S *,2 R *)-1,2-Dihydroxypentyl]-4-methoxy-5,6-dihydro-2 H -pyran-2-one(2013-10) Valenti, Domenic J.; Arif, Atta M.; Strobel, Gary A.; Harper, James K.The title compound, C11H18O5, was isolated from a liquid culture of Pestalotiopsis sp. In the molecule, the pyran-2-one ring assumes a half-chair conformation. The two terminal C atoms of the pentyl group were refined as disordered over two sets of sites, with refined occupancies of 0.881 (10) and 0.119 (10). In the crystal, molecules are linked via O-H...O hydrogen bonds forming a three-dimensional network.