Scholarly Work - Civil Engineering

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    Pharmaceutical impacts on aerobic granular sludge morphology and potential implications for abiotic removal
    (Elsevier, 2024-02) Bodle, Kylie B.; Kirkland, Catherine M.
    The goal of this study was to investigate abiotic pharmaceutical removal and abiotic pharmaceutical effects on aerobic granular sludge morphology. For 80 days, a pharmaceutical mixture containing approximately 150 μg/L each of diclofenac, erythromycin, and gemfibrozil was fed to an aerobic granular sludge sequencing batch reactor and granule characteristics were compared with those from a control reactor. Aqueous and solid phase pharmaceutical concentrations were monitored and staining was used to assess changes in biofilm structures. Solid phase pharmaceutical concentrations were elevated over the first 12 days of dosing; however, they then dropped, indicative of desorption. The lipid content in pharmaceutical-exposed granules declined by approximately half over the dosing period, though the relative concentrations of other key biofilm components (proteins, alpha-, and beta-polysaccharides) did not change. Batch experiments were conducted to try to find an explanation for the desorption observed, but reduced solid phase pharmaceutical concentrations could not be linked with the presence of common wastewater constituents such as ammonia or phosphate. Sorption of all three compounds was modeled best by the Henry isotherm, indicating that, even at 150 μg/L, granules’ sorption site coverage was incomplete. Altogether, this study demonstrates that simplified batch systems may not accurately represent the complex abiotic processes occurring in flow-through, biotic systems.
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    Treatment performance and microbial community structure in an aerobic granular sludge sequencing batch reactor amended with diclofenac, erythromycin, and gemfibrozil
    (Frontiers Media SA, 2023-09) Bodle, Kylie B.; Mueller, Rebecca C.; Pernat, Madeline R.; Kirkland, Catherine M.
    This study characterizes the effects of three commonly detected pharmaceuticals—diclofenac, erythromycin, and gemfibrozil—on aerobic granular sludge. Approximately 150 µg/L of each pharmaceutical was fed in the influent to a sequencing batch reactor for 80 days, and the performance of the test reactor was compared with that of a control reactor. Wastewater treatment efficacy in the test reactor dropped by approximately 30-40%, and ammonia oxidation was particularly inhibited. The relative abundance of active Rhodocyclaceae, Nitrosomonadaceae, and Nitrospiraceae families declined throughout exposure, likely explaining reductions in wastewater treatment performance. Pharmaceuticals were temporarily removed in the first 12 days of the test via both sorption and degradation; both removal processes declined sharply thereafter. This study demonstrates that aerobic granular sludge may successfully remove pharmaceuticals in the short term, but long-term tests are necessary to confirm if pharmaceutical removal is sustainable.
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    Flow-Control Plates to Manage Denil Fishways in Irrigation Diversions for Upstream Passage of Arctic Grayling
    (U.S. Fish and Wildlife Service, 2023-06) Plymesser, Kathryn; Blue, Tyler; Kappenman, Kevin M.; Blank, Matthew; Cahoon, Joel; Dockery, David
    Small-stream irrigation diversions are key elements of many on-farm irrigation systems but can act as barriers to aquatic species. Denil fishways have been installed at irrigation diversion structures throughout the Big Hole River watershed in Montana to provide upstream passage for a population of Arctic Grayling Thymallus arcticus. When stream flows are low and irrigation demand is high, irrigators look for ways to maintain adequate diversion, but doing so may reduce the effectiveness of the fishways. In response, agencies and irrigators have proposed flow-control plates placed at the upstream end of fishways. We conducted laboratory-based fishway efficiency experiments with Arctic Grayling placed in an open-channel flume fitted with a Denil fishway and three flow plates. Of the total 200 fish that we used, the fishway entrance attracted 154 fish and we counted these fish as participants. We operated the fishway under varying flow conditions using three flow-control plate treatments and a control to investigate 1) the extent to which each treatment reduced flow compared to the control, and 2) the extent to which each treatment impacted passage success of Arctic Grayling relative to the control. We measured passage success as the ratio of the number of fish that fully ascended the fishway treatment to the number of participant fish attracted to the fishway treatment. One of the three plates, the Denil slot treatment, showed no evidence of reducing either flow or passage success. Another plate, the standard treatment, showed no evidence of reducing flow but moderate evidence of reducing passage success (P = 0.03). The only treatment to significantly reduce water flow rate was the narrowed Denil slot treatment and there was no evidence this treatment reduced passage in comparison to the control. Over all trials, water flow rate through the Denil fishway had a strong positive influence on fish passage success.
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    Impact of deicing salts on pervious concrete pavement
    (Frontiers Media SA, 2023-06) Feng, Lichao; Zhang, Yongran; Wang, Xiaowei; Mery, Stephene; Akin, Michelle; Li, Mengchao; Xie, Ning; Li, Zhenming; Shi, Xianming
    Two pervious concrete projects (named as SR28 and SR431), with the same mixture design but different winter maintenance activities, were included in this research. Both projects are located in the Lake Tahoe area, Nevada, United States. Testing results indicated that the mechanical properties were significantly higher in SR28 cored samples than the ones in SR431. It was found that the SR28 pieces have fewer air voids, while the SR431 samples have higher water absorption and hydraulic conductivity, and the SR28 samples show fare better performance against repeated freezing and thawing cycles than the SR431 ones. scanning electron microscope pictures of crack surfaces in cores taken from SR28 indicate that the cement binder phase has been largely retained. However, in the coring sample of SR431, needle-shaped residues can be seen within the cement binder phase and an abundance of precipitated micro-sized crystalized particles can be observed. On a micrometer scale, the μCT examination reveals that the porosity of SR28 samples is significantly less than that of SR431. The analyzing results give a clue to demonstrate the durability of pervious concrete pavement can be attributed to the construction quality control, maintenance activity, or the weather and locations of the field sites.
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    High-Level Assessment ofStatewide GNSS-RTN Business Models
    (2023-06) Al-Kaisy, Ahmed; Raza, Sajid
    The applications of geospatial technologies and positioning data embrace every sphere of modern-day science and industry where geographical positioning matters. Among all other fields, geospatial technology plays a remarkable role in the transportation sector and has the potential to play an even more critical role in future autonomous transportation systems. In this regard, the GNSS-Real-Time Network (GNSS-RTN) technology is promising in meeting the needs of automation in most advanced transportation applications. The GNSS-RTN is a satellite-based positioning system that uses a network of reference stations to provide centimeter-level accuracy in positioning data in real-time. The technical aspect and working technology of GNSS-RTN are widely studied, however, only limited research has been conducted on the various GNSS-RTN business models currently in use nationally and internationally. Therefore, this study aims at assessing the various GNSS-RTN business models currently used in practice as well as those that are deemed potentially viable but have not yet moved to practice. Eight different business models were cataloged and used in the current assessment. All business models were assessed using three criteria: state control, sustainability, and state/agency costs. The findings of this research are important in helping state agencies make informed decisions as they build, expand or manage their own GNSS-RTN systems.
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    Pharmaceutical Sorption to Lab Materials May Overestimate Rates of Removal in Lab-Scale Bioreactors
    (Springer Science and Business Media LLC, 2022-11) Bodle, Kylie B.; Pernat, Madeline R.; Kirkland, Catherine M.
    Environmental contamination from pharmaceuticals has received increased attention from researchers in the past 20 years. As such, numerous lab-scale studies have sought to characterize the effects of these contaminants on various targets, as well as determine improved removal methods. Many studies have used lab-scale bioreactors to investigate pharmaceutical effects on wastewater bacteria, as wastewater treatment plants often act as reservoirs for pharmaceuticals. However, few—if any—of these studies report the specific lab materials used during testing, such as tubing or pipette tip type. In this study, the pharmaceuticals erythromycin, diclofenac, and gemfibrozil were exposed to different micropipette tips, syringe filters, and tubing types, and losses over time were evaluated. Losses to tubing and syringe filters were particularly significant and neared 100%, depending on the pharmaceutical compound and length of exposure time. Results discussed herein indicate that pharmaceutical sorption to various lab supplies results in decreases to both dosed and quantified pharmaceutical concentrations. Studies that fail to consider this source of loss may therefore draw inaccurate conclusions about pharmaceutical effects or removal efficiencies.
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    Mapping liquid water content in snow at the millimeter scale: an intercomparison of mixed-phase optical property models using hyperspectral imaging and in situ measurements
    (Copernicus Publications, 2022-01) Donahue, Christopher; Skiles, S. McKenzie; Hammonds, Kevin
    It is well understood that the distribution and quantity of liquid water in snow is relevant for snow hydrology and avalanche forecasting, yet detecting and quantifying liquid water in snow remains a challenge from the micro- to the macro-scale. Using near-infrared (NIR) spectral reflectance measurements, previous case studies have demonstrated the capability to retrieve surface liquid water content (LWC) of wet snow by leveraging shifts in the complex refractive index between ice and water. However, different models to represent mixed-phase optical properties have been proposed, including (1) internally mixed ice and water spheres, (2) internally mixed water-coated ice spheres, and (3) externally mixed interstitial ice and water spheres. Here, from within a controlled laboratory environment, we determined the optimal mixed-phase optical property model for simulating wet snow reflectance using a combination of NIR hyperspectral imaging, radiative transfer simulations (Discrete Ordinate Radiative Transfer model, DISORT), and an independent dielectric LWC measurement (SLF Snow Sensor). Maps of LWC were produced by finding the lowest residual between measured reflectance and simulated reflectance in spectral libraries, generated for each model with varying LWC and grain size, and assessed against the in situ LWC sensor. Our results show that the externally mixed model performed the best, retrieving LWC with an uncertainty of ∼1 %, while the simultaneously retrieved grain size better represented wet snow relative to the established scaled band area method. Furthermore, the LWC retrieval method was demonstrated in the field by imaging a snowpit sidewall during melt conditions and mapping LWC distribution in unprecedented detail, allowing for visualization of pooling water and flow features.
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    Test of 90-Foot Post-Tensioned Concrete Girder with Unbonded Tendons
    (American Concrete Institute, 2021-09) Pujol, Santiago; Fick, Damon; Fargier-Gabaldón, Luis B.
    A full-scale post-tensioned concrete girder with unbonded tendons was tested to investigate whether, under vertical forces, a full flexural mechanism (with three hinging regions) would form, and what strand stress would be reached in that condition. The specimen was a 0.91 m (3 ft) deep T-beam with a 2.43 m (8 ft) wide flange, spanning over two supports spaced at 18.3 m (60 ft) with two 4.6 m (15 ft) cantilevers and featured a parabolic tendon profile. Transverse reinforcement to resist shear and longitudinal “mild” reinforcement were also provided. A uniformly distributed load was applied on the main span and concentrated loads were applied to the ends of the cantilevers. While the main span was loaded, the two concentrated loads on the cantilevers provided a reaction force to minimize rotations at supports. At the end of the test, the girder deflected 278 mm (10.9 in., L/65) and carried 231 kN/m (15.5 kip/ft) over the main span. A full plastic mechanism formed with hinging regions at supports and at midspan. Test results suggest the unbonded tendons nearly reached their nominal strength (fpu) and that a limit analysis is adequate for estimating the flexural strength of comparable post-tensioned girders.
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    International Journal of Multiphase Flow
    (Elsevier BV, 2021-07) Tagliavini, Giorgia; McCorquodale, Mark; Westbrook, Chris; Corso, Pascal; Krol, Quirine; Holzner, Markus
    This study considers complex ice particles falling in the atmosphere: predicting the drag of such particles is important for developing of climate models parameterizations. A Delayed-Detached Eddy Simulation model is developed to predict the drag coefficient of snowflakes falling at Reynolds number between 50 and 2200. We first consider the case where the orientation of the particle is known a posteriori, and evaluate our results against laboratory experiments using 3D-printed particles of the same shape, falling at the same Reynolds number. Close agreement is found in cases where the particles fall stably, while a more complex behavior is observed in cases where the flow is unsteady. The second objective of this study is to evaluate methods for estimating the drag coefficient when the orientation of the particles is not known a posteriori. We find that a suitable average of two orientations corresponding to the minimum and maximum eigenvalues of the inertia tensor provides a good estimate of the particle drag coefficient. Meanwhile, existing correlations for the drag on non-spherical particles produce large errors (≈50%). A new formula to estimate snow particles settling velocity is also proposed. Our approach provides a framework to investigate the aerodynamics of complex snowflakes and is relevant to other problems that involve the sedimentation of irregular particles in viscous fluids.
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    Empirical Bayes application on low-volume roads: Oregon case study
    (Elsevier BV, 2021-12) Al-Kaisy, Ahmed; Huda, Kazi Tahsin
    ntroduction: This paper investigates the Empirical Bayes (EB) method and the Highway Safety Manual (HSM) predictive methodology for network screening on low-volume roads in Oregon. Method: A study sample of around 870 miles of rural two-lane roadways with extensive crash, traffic and roadway information was used in this investigation. To understand the effect of low traffic exposure in estimating the EB expected number of crashes, the contributions of both the observed and the HSM predicted number of crashes were analyzed. Results and Conclusions: The study found that, on low-volume roads, the predicted number of crashes is the major contributor in estimating the EB expected number of crashes. The study also found a large discrepancy between the observed and the predicted number of crashes using the HSM procedures calibrated for the state of Oregon, which could partly be attributed to the unique attributes of low-volume roads that are different from the rest of the network. However, the expected number of crashes for the study sample using the HSM EB method was reasonably close to the observed number of crashes over the 10-year study period. Practical Applications: Based on the findings, it can still be very effective to use network screening methods that rely primarily on risk factors for low-volume road networks. This is especially applicable in situations where accurate and reliable crash data are not available.
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    Reanalysis of Polythermal Glacier Thermal Structure Using Radar Diffraction Focusing
    (American Geophysical Union, 2022-01) Delf, Richard; Bingham, Robert G.; Curtis, Andrew; Singh, Satyan; Giannopoulos, Antonios; Schwarz, Benjamin; Borstad, Chris P.
    Ground-penetrating radar (GPR) is widely used on polythermal glaciers to image bed topography and detect internal scatter due to water inclusions in temperate ice. The glaciological importance of this is twofold: bed topography is a primary component for modeling the long-term evolution of glaciers and ice sheets, and the presence of temperate ice and associated englacial water significantly reduces overall ice viscosity. Englacial water has a direct influence on radar velocity, which can result in incorrect observations of bed topography due to errors in depth conversion. Assessment of radar velocities often requires multi-offset surveys, yet these are logistically challenging and time consuming to acquire, hence techniques to extract velocity from common-offset data are required. We calculate englacial radar velocity from common offset GPR data collected on Von Postbreen, a polythermal glacier in Svalbard. We first separate and enhance the diffracted wavefield by systematically assessing data coherence. We then use the focusing metric of negative entropy to deduce a migration velocity field and produce a velocity model which varies spatially across the glacier. We show that this velocity field successfully differentiates between areas of cold and temperate ice and can detect lateral variations in radar velocity close to the glacier bed. This velocity field results in consistently lower ice depths relative to those derived from a commonly assumed constant velocity, with an average difference of 4.9 ± 2.5% of local ice depth. This indicates that diffraction focusing and velocity estimation are crucial in retrieving correct bed topography in the presence of temperate ice.
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    The Use of Fiber-Reinforced Polymers in Wildlife Crossing Infrastructure
    (MDPI, 2020-02) Bell, Matthew; Fick, Damon; Ament, Rob; Lister, Nina-Marie
    The proven effectiveness of highway crossing infrastructure to mitigate wildlife-vehicle collisions with large animals has made it a preferred method for increasing motorist and animal safety along road networks around the world. The crossing structures also provide safe passage for small- and medium-sized wildlife. Current methods to build these structures use concrete and steel, which often result in high costs due to the long duration of construction and the heavy machinery required to assemble the materials. Recently, engineers and architects are finding new applications of fiber-reinforced polymer (FRP) composites, due to their high strength-to-weight ratio and low life-cycle costs. This material is better suited to withstand environmental elements and the static and dynamic loads required of wildlife infrastructure. Although carbon and glass fibers along with new synthetic resins are most commonly used, current research suggests an increasing incorporation and use of bio-based and recycled materials. Since FRP bridges are corrosion resistant and hold their structural properties over time, owners of the bridge can benefit by reducing costly and time-consuming maintenance over its lifetime. Adapting FRP bridges for use as wildlife crossing structures can contribute to the long-term goals of improving motorist and passenger safety, conserving wildlife and increasing cost efficiency, while at the same time reducing plastics in landfills.
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    Swimming Performance of Rainbow Trout and Westslope Cutthroat Trout in an Open-Channel Flume
    (2020-06) Blank, Matt D.; Kappenman, Kevin M.; Plymesser, Kathryn; Banner, Katharine M.; Cahoon, Joel
    We used an open-channel flume to characterize the swimming performance of Rainbow Trout Oncorhynchus mykiss and Westslope Cutthroat Trout Oncorhynchus clarki lewisi ranging nominally in fork length from 15 to 30 cm. With an open-channel flume, we observed volitional swim performance of wild-caught Rainbow Trout and Westslope Cutthroat Trout; the fish were not coerced, prodded, or spooked into action. We also observed the maximum short-duration swim speed of the fish, providing important effective leap or velocity challenge information for the design of intentional barriers. We conducted the experiment with a consistently low water velocity challenge and characterized swim speeds by using weighted least-squares regression, revealing no evidence of a difference in swim speeds between the two species. We estimated the overall average swim speed for Rainbow Trout to be 0.84 m/s (SE = 0.02), with a 95% confidence interval of 0.79–0.89 m/s, and that for Westslope Cutthroat Trout to be 0.84 m/s (SE = 0.03), with a 95% confidence interval of 0.78–0.90 m/s. The maximum swim speeds observed were 2.72 m/s for Rainbow Trout and 3.55 m/s for Westslope Cutthroat Trout. The project results provide new information on the swimming ability of wild Rainbow Trout and Westslope Cutthroat Trout that can be used to improve fish passage or barrier design.
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    Biomineralization of Plastic Waste to Improve the Strength of Plastic-Reinforced Cement Mortar
    (2021-04) Kane, Seth; Thane, Abby; Espinal, Michael; Lunday, Kendra; Armagan, Hakan; Phillips, Adrienne J.; Heveran, Chelsea M.; Ryan, Cecily A.
    The development of methods to reuse large volumes of plastic waste is essential to curb the environmental impact of plastic pollution. Plastic-reinforced cementitious materials (PRCs), such as plastic-reinforced mortar (PRM), may be potential avenues to productively use large quantities of low-value plastic waste. However, poor bonding between the plastic and cement matrix reduces the strength of PRCs, limiting its viable applications. In this study, calcium carbonate biomineralization techniques were applied to coat plastic waste and improved the compressive strength of PRM. Two biomineralization treatments were examined: enzymatically induced calcium carbonate precipitation (EICP) and microbially induced calcium carbonate precipitation (MICP). MICP treatment of polyethylene terephthalate (PET) resulted in PRMs with compressive strengths similar to that of plastic-free mortar and higher than the compressive strengths of PRMs with untreated or EICP-treated PET. Based on the results of this study, MICP was used to treat hard-to-recycle types 3–7 plastic waste. No plastics investigated in this study inhibited the MICP process. PRM samples with 5% MICP-treated polyvinyl chloride (PVC) and mixed type 3–7 plastic had compressive strengths similar to plastic-free mortar. These results indicate that MICP treatment can improve PRM strength and that MICP-treated PRM shows promise as a method to reuse plastic waste.
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    Hydraulic Analysis at the Interface of the Yellowstone River and the Huntley, Montana Irrigation Diversion Fish Bypass
    (Montana State University, 2021-11) Johnson, Andrew; Cahoon, Joel; Zale, Al; Plymesser, Katey; Blank, Matthew
    The nature-like bypass channel built to allow fish to circumvent the Huntley Diversion Dam on the Yellowstone River was constructed in 2015. A project was commissioned in 2019 to determine the effectiveness of the bypass using hydraulic modeling and fish detection techniques. During the course of the study it was observed that there may be a localized zone of high water velocity at the interface between the upstream end of the bypass and the main channel of the river -an area just upstream of the low-head dam. The concern this raises is that some fish that successfully negotiate the bypass channel may be returned directly over the dam due a difficult hydraulic condition at the interface. That observation prompted a more focused hydraulic modeling exercise as reported herein. A detailed 2-D HEC-RAS model was developed to investigate the hydraulic conditions. The model predicts localized water velocities of up to 15 ft/sec. At low river flows there appears to be adequate pathways for fish to avoid this high velocity region, but as river flow increases so does the area in which the velocity is high. From these results it is likely that, during higher river flow periods, the bypass channel may be passable, but fish may struggle to re-enter the river channel successfully. Suggested physical alterations to the site to help overcome this range from the addition of large rip rap to rerouting the upstream end of the bypass channel. Acknowledgement
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    In Situ Effective Snow Grain Size Mapping Using a Compact Hyperspectral Imager
    (2021-02) Donahue, Christopher; Skiles, S. McKenzie; Hammonds, Kevin
    Effective snow grain radius (re) is mapped at high resolution using near-infrared hyperspectral imaging (NIR-HSI). The NIR-HSI method can be used to quantify re spatial variability, change in re due to metamorphism, and visualize water percolation in the snowpack. Results are presented for three different laboratory-prepared snow samples (homogeneous, ice lens, fine grains over coarse grains), the sidewalls of which were imaged before and after melt induced by a solar lamp. The spectral reflectance in each ~3 mm pixel was inverted for re using the scaled band area of the ice absorption feature centered at 1030 nm, producing re maps consisting of 54 740 pixels. All snow samples exhibited grain coarsening post-melt as the result of wet snow metamorphism, which is quantified by the change in re distributions from pre- and post-melt images. The NIR-HSI method was compared to re retrievals from a field spectrometer and X-ray computed microtomography (micro-CT), resulting in the spectrometer having the same mean re and micro-CT having 23.9% higher mean re than the hyperspectral imager. As compact hyperspectral imagers become more widely available, this method may be a valuable tool for assessing re spatial variability and snow metamorphism in field and laboratory settings.
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    Hydraulic characterization and removal of metals and nutrients in an aerated horizontal subsurface flow “racetrack” wetland treating oil industry effluent
    (Elsevier, 2021-07) Mozaffari, Mohammad-Hosein; Shafiepour, Ehsan; Mirbagheri, Seyed Ahmad; Rakhshandehroo, Gholamreza; Wallace, Scott; Stefanakis, Alexandros I.
    Constructed wetlands (CW) are an attractive technology due to their operational simplicity and low life-cycle cost. It has been applied for refinery effluent treatment but mostly single-stage designs (e.g., vertical or horizontal flow) have been tested. However, to achieve a good treatment efficiency for industrial effluents, different treatment conditions (both aerobic and anaerobic) are needed. This means that hybrid CW systems are typically required with a respectively increased area demand. In addition, a strong aerobic environment that facilitates the formation of iron, manganese, zinc and aluminum precipitates cannot be established with passive wetland systems, while the role of these oxyhydroxide compounds in the further co-precipitation and removal of heavy metals such as copper, nickel, lead, and chromium that can simplify the overall treatment of industrial wastewaters is poorly understood in CW. Therefore, this study tests for the first time an innovative CW design that combines an artificially aerated section with a non-aerated section in a single unit applied for oil refinery wastewater treatment. Four pilot units were tested with different design (i.e., planted/unplanted, aerated/non-aerated) and operational (two different hydraulic loading rates) characteristics to estimate the role of plants and artificial aeration and to identify the optimum design configuration. The pilot units received a primary refinery effluent, i.e., after passing through a dissolved air flotation unit. The first-order removal of heavy metals under aerobic conditions is evaluated, along with the removal of phenols and nutrients. High removal rates for Fe (96–98%), Mn (38–81%), Al (49–73%), and Zn (99–100%) generally as oxyhydroxide precipitates were found, while removal of Cu (61–80%), Ni (70–85%), Pb (96–99%) and Cr (60–92%) under aerobic conditions was also observed, likely through co-precipitation. Complete phenols and ammonia nitrogen removal was also found. The first-order rate coefficient (k) calculated from the collected data demonstrates that the tested CW represents an advanced wetland design reaching higher removal rates at a smaller area demand than the common CW systems.
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    Variation of Small-Strain Shear Modulus of Unsaturated Silt under Successive Cycles of Drying and Wetting
    (2020-07) Khosravi, Ali; Hashemi, Amirhossein; Ghadirianniari, Sahar; Khosravi, Mohammad
    A new framework is developed to extend an existing small-strain shear modulus (𝐺max) model to determine 𝐺max of unsaturated silty soils along different paths of the soil water retention curve (SWRC) including the scanning loops. The suitability of the proposed framework is validated against experimental results of a series of bender-element tests performed in this study and data reported in literature. Measured values of 𝐺max showed a slight hysteresis in the 𝐺max measurements along the scanning curves of the SWRC, with lower values along the wetting scanning paths. However, results indicated that the value of 𝐺max was recovered once the main drying path was reached. Results of this study also indicated that 𝐺max behavior of the silty specimen along the scanning curves was stress dependent. The model was observed to follow the experimental data along different paths of the SWRC including scanning curves.
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    Kinetics of Calcite Precipitation by Ureolytic Bacteria under Aerobic and Anaerobic Conditions
    (2019-05) Mitchell, Andrew C.; Espinosa-Ortiz, Erika J.; Parks, Stacy L.; Phillips, Adrienne J.; Cunningham, Alfred B.; Gerlach, Robin
    The kinetics of urea hydrolysis (ureolysis) and induced calcium carbonate (CaCO3) precipitation for engineering use in the subsurface was investigated under aerobic conditions using Sporosarcina pasteurii (ATCC strain 11859) as well as Bacillus sphaericus strains 21776 and 21787. All bacterial strains showed ureolytic activity inducing CaCO3 precipitation aerobically. Rate constants not normalized to biomass demonstrated slightly higher-rate coefficients for both ureolysis (kurea) and CaCO3 precipitation (kprecip) for B. sphaericus 21776 (kurea=0.10±0.03 h−1, kprecip=0.60±0.34 h−1) compared to S. pasteurii (kurea=0.07±0.02 h−1, kprecip=0.25±0.02 h−1), though these differences were not statistically significantly different. B. sphaericus 21787 showed little ureolytic activity but was still capable of inducing some CaCO3 precipitation. Cell growth appeared to be inhibited during the period of CaCO3 precipitation. Transmission electron microscopy (TEM) images suggest this is due to the encasement of cells and was reflected in lower kurea values observed in the presence of dissolved Ca. However, biomass regrowth could be observed after CaCO3 precipitation ceased, which suggests that ureolysis-induced CaCO3 precipitation is not necessarily lethal for the entire population. The kinetics of ureolysis and CaCO3 precipitation with S. pasteurii was further analyzed under anaerobic conditions. Rate coefficients obtained in anaerobic environments were comparable to those under aerobic conditions; however, no cell growth was observed under anaerobic conditions with NO−3, SO2−4 or Fe3+ as potential terminal electron acceptors. These data suggest that the initial rates of ureolysis and ureolysis-induced CaCO3 precipitation are not significantly affected by the absence of oxygen but that long-term ureolytic activity might require the addition of suitable electron acceptors. Variations in the ureolytic capabilities and associated rates of CaCO3 precipitation between strains must be fully considered in subsurface engineering strategies that utilize microbial amendments.
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    Using hyperspectral plant signatures for CO2 leak detection during the 2008 ZERT CO2 sequestration field experiment in Bozeman, MT
    (2010-03) Male, Erin J.; Pickles, William L.; Silver, Eli A.; Hoffmann, Gary D.; Lewicki, Jennifer; Apple, Martha E.; Repasky, Kevin S.; Burton, Elizabeth A.
    Hyperspectral plant signatures can be used as a short-term, as well as long-term (100-year timescale) monitoring technique to verify that CO2 sequestration fields have not been compromised. An influx of CO2 gas into the soil can stress vegetation, which causes changes in the visible to near-infrared reflectance spectral signature of the vegetation. For 29 days, beginning on July 9, 2008, pure carbon dioxide gas was released through a 100-m long horizontal injection well, at a flow rate of 300 kg day−1. Spectral signatures were recorded almost daily from an unmown patch of plants over the injection with a “FieldSpec Pro” spectrometer by Analytical Spectral Devices, Inc. Measurements were taken both inside and outside of the CO2 leak zone to normalize observations for other environmental factors affecting the plants. Four to five days after the injection began, stress was observed in the spectral signatures of plants within 1 m of the well. After approximately 10 days, moderate to high amounts of stress were measured out to 2.5 m from the well. This spatial distribution corresponded to areas of high CO2 flux from the injection. Airborne hyperspectral imagery, acquired by Resonon, Inc. of Bozeman, MT using their hyperspectral camera, also showed the same pattern of plant stress. Spectral signatures of the plants were also compared to the CO2 concentrations in the soil, which indicated that the lower limit of soil CO2 needed to stress vegetation is between 4 and 8% by volume.
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