Scholarly Work - Research Centers
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Item Discrimination of herbicide-resistant kochia with hyperspectral imaging(2018-03) Nugent, Paul W.; Shaw, Joseph A.; Jha, Prashant; Scherrer, Bryan; Donelick, Andrew; Kumar, VipanA hyperspectral imager was used to differentiate herbicide-resistant versus herbicide-susceptible biotypes of the agronomic weed kochia, in different crops in the field at the Southern Agricultural Research Center in Huntley, Montana. Controlled greenhouse experiments showed that enough information was captured by the imager to classify plants as either a crop, herbicidesusceptible or herbicide-resistant kochia. The current analysis is developing an algorithm that will work in more uncontrolled outdoor situations. In overcast conditions, the algorithm correctly identified dicamba-resistant kochia, glyphosate-resistant kochia, and glyphosate-and dicamba-susceptible kochia with 67%, 76%, and 80% success rates, respectively. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.Item First report of Ser653 Asn mutation endowing high-level resistance to imazamox in downy brome (Bromus tectorum L.)(2017-08) Kumar, Vipan; Jha, PrashantBACKGROUND Bromus tectorum L. is one of the most troublesome grass weed species in cropland and non-cropland areas of the northwestern United States. In summer 2016, a B. tectroum accession (R) that survived imazamox at the field-use rate (44 g ha-1 ) in an imidazolinone-tolerant (IMI-tolerant or Clearfieldâ„¢ ) winter wheat field was collected near Hammond, Carter County, MT, USA. The aim of this study was to determine the resistance profile of the B. tectroum R accession to imazamox and other ALS inhibitors, and investigate the mechanism of resistance to imazamox. RESULTS The R B. tectorum accession had a high-level resistance (110.1-fold) to imazamox (IMI) and low to moderate-levels cross-resistance to pyroxsulam (TP) (4.6-fold) and propoxycarbazone (SCT) (13.9-fold). The R accession was susceptible to sulfosulfuron (SU) and quizalofop and clethodim (ACCase inhibitors), paraquat (PS I inhibitor), glyphosate (EPSPS inhibitor), and glufosinate (GS inhibitor). Sequence analysis of the ALS gene revealed a single, target-site Ser653 Asn mutation in R plants. The pre-treatment of malathion followed by imazamox at 44 or 88 g ha-1 did not reverse the resistance phenotype. CONCLUSION This is the first report of evolution of cross-resistance to ALS-inhibiting herbicides in B. tectorum. A single-point mutation, Ser653 Asn, was identified, conferring the high-level resistance to imazamox.Item Influence of Nitrogen Rate, Seeding Rate, and Weed Removal Timing on Weed Interference in Barley and Effect of Nitrogen on Weed Response to Herbicides(2017-01) Kumar, Vipan; Jha, PrashantField experiments were conducted at the Montana State University Southern Agricultural Research Center, Huntley, MT, in 2011 through 2013 to determine the effect of nitrogen (N) rate, seeding rate, and weed removal timing on weed interference in barley. A delay in weed removal timing from the 3- to 4-leaf (LF) stage to the 8- to 10-LF stage of barley resulted in up to 3.5-fold increase in total weed biomass and 10% reduction in barley biomass, and this was unaffected by a N rate that ranged from 56 (low) to 168 (high) kg ha^sup -1^. The effect of N rate on barley biomass was more pronounced when weed removal was delayed from the 3- to 4-LF stage to the 8- to 10-LF stage of barley and in nontreated plots. Increasing the barley seeding rate from 38 to 152 kg ha^sup -1^ increased the barley plant density by 50%, biomass by 13%, and grain yield by 29%, averaged over N rates and weed removal timing. On the basis of 5 and 10% levels of acceptable yield loss, the addition of ≥112 kg N ha^sup -1^ delayed the critical timing of weed removal by at least 1.3 wk in barley, compared with the 56 kg N ha^sup -1^ rate. A medium or high N rate prevented reduction in barley grain quality (plumpness and test weight) observed when the seeding rate was increased from 38 to 76 or 152 kg ha^sup -1^ at the low N rate. In a separate greenhouse study, the effect of N rate on the effectiveness of various herbicides for controlling wild oat, green foxtail, kochia, or Russian thistle was investigated. Results highlighted that wild oat or green foxtail grown under 56 kg N ha^sup -1^ (low N) soil required 1.4 to 2.6 times higher doses of clodinafop, fenoxaprop, flucarbazone, glyphosate, glufosinate, pinoxaden, or tralkoxydim for 50% reduction in shoot dry weights (GR^sub 50^) compared with plants grown under 168 kg N ha^sup -1^ (high N). Similarly, a reduced efficacy of thifensulfuron methyl + tribenuron methyl, metsulfuron methyl, or bromoxynil + pyrasulfotole was observed (evident from the GR^sub 50^ values) for kochia or Russian thistle grown under low- vs. high-N soil. Information gained from this research will aid in developing cost-effective, integrated weed management (IWM) strategies in cereals and in educating growers on the importance of fertilizer N management as a component of IWM programs.Item Weed management using crop competition in the United States: A review(2016-07) Jha, Prashant; Kumar, Vipan; Godara, Rakesh K; Chauhan, Bhagirath S.Exploiting the competitive ability of crops is essential to develop cost-effective and sustainable weed management practices. Reduced row spacing, increased seeding rates, and selection of competitive cultivars can potentially manage crop-weed competition in cotton, soybean, wheat, and corn. These cultural weed management practices facilitate a more rapid development of crop canopy that adversely affect the emergence, density, growth, biomass, and subsequently the seed production of weeds during a growing season. These cultural practices can also favour the weed suppressive ability of the crop by influencing the canopy architecture traits (plant height, canopy density, leaf area index, rate of leaf area development, and leaf distribution). These crop-competition attributes can potentially reduce the risk of crop yield losses due to interference from weed cohorts that escape an early- or a late-season post-emergence herbicide application. Furthermore, reduced row spacing, increased seeding rates, and weed-competitive cultivars are effective in reducing reliance on a single site-of-action herbicides, thereby reducing the selection pressure for development of herbicide-resistant weed populations in a cropping system.Item Molecular Basis of Evolved Resistance to Glyphosate & Acetolactate Synthase-Inhibitor Herbicides in Kochia (Kochia scoparia) Accessions from Montana(2015-10) Kumar, Vipan; Jha, Prashant; Giacomini, Darci; Westra, Eric P.; Westra, PhilipThe rapid evolution and spread of glyphosate-resistant (GR) kochia in the Northern Great Plains is an increasing threat to GR cropping systems and conservation tillage practices common in this region. GR kochia accessions with 4.6- to 11-fold levels of resistance to glyphosate have recently been reported in Montana. Those GR kochia accessions were also suspected to be resistant to acetolactate synthase (ALS) inhibitors, i.e., multiple herbicide-resistant (MHR) kochia. In this research, the level of resistance to the ALS-inhibitor herbicides (sulfonylureas) and the molecular mechanisms conferring resistance to glyphosate and ALS-inhibitor herbicides in MHR kochia was investigated. On the basis of whole-plant dose–response assays, MHR kochia accessions (GIL01, JOP01, and CHES01) were 9.3- to 30-fold more resistant to premixed thifensulfuron methyl + tribenuron methyl + metsulfuron methyl than the susceptible (SUS) accession. In an in vivo leaf-disk shikimate assay, MHR plants accumulated less shikimate than the SUS plants at a discriminate dose of 100 μM glyphosate. Sequencing of the conserved region of EPSPS revealed no target-site mutation at Thr102 or Pro106 residue. MHR kochia accessions had increased relative EPSPS gene copies (~ 4 to 10) compared with the SUS accession (single copy). Furthermore, MHR kochia accumulated higher EPSPS protein compared with the SUS plants. Resistance to the ALS-inhibitor herbicides was conferred by Pro197 amino acid substitution (proline to glutamine). EPSPS gene amplification and a single target-site mutation at Pro197 in ALS gene confer resistance to glyphosate and ALS-inhibitor herbicides, respectively, in MHR kochia accessions from Montana. This is the first confirmation of occurrence of MHR kochia in Montana.Item Growth & Reproduction of Glyphosate-Resistant & Susceptible Populations of Kochia scoparia(2015-11) Kumar, Vipan; Jha, PrashantEvolution of glyphosate-resistant kochia is a threat to no-till wheat-fallow and glyphosate-resistant (GR) cropping systems of the US Great Plains. The EPSPS (5-enol-pyruvylshikimate-3-phosphate synthase) gene amplification confers glyphosate resistance in the tested Kochia scoparia (L.) Schrad populations from Montana. Experiments were conducted in spring to fall 2014 (run 1) and summer 2014 to spring 2015 (run 2) to investigate the growth and reproductive traits of the GR vs. glyphosate-susceptible (SUS) populations of K. scoparia and to determine the relationship of EPSPS gene amplification with the level of glyphosate resistance. GR K. scoparia inbred lines (CHES01 and JOP01) exhibited 2 to 14 relative copies of the EPSPS gene compared with the SUS inbred line with only one copy. In the absence of glyphosate, no differences in growth and reproductive parameters were evident between the tested GR and SUS inbred lines, across an intraspecific competition gradient (1 to 170 plants m-2). GR K. scoparia plants with 2 to 4 copies of the EPSPS gene survived the field-use rate (870 g ha-1) of glyphosate, but failed to survive the 4,350 g ha-1 rate of glyphosate (five-times the field-use rate). In contrast, GR plants with 5 to 14 EPSPS gene copies survived the 4,350 g ha-1 of glyphosate. The results from this research indicate that GR K. scoparia with 5 or more EPSPS gene copies will most likely persist in field populations, irrespective of glyphosate selection pressure.Item Tank Mixing Pendimethalin with Pyroxasulfone and Chloroacetamide Herbicides Enhances In-Season Residual Weed Control in Corn(2015-05) Jha, Prashant; Kumar, Vipan; Garcia, Josefina; Reichard, NicholasKochia, common lambsquarters, and wild buckwheat are major problem weeds in glyphosate-resistant corn production in the northern Great Plains of the United States. Field research was conducted in 2011 and 2012 near Huntley, MT to investigate effective PRE herbicides applied alone or in premixes with or without tank-mixed pendimethalin for extended in-season residual control of the selected broadleaf weeds in glyphosate-resistant corn. Control of kochia, common lambsquarters, and wild buckwheat with recently registered herbicide premixes, including saflufenacil + dimethenamid-P and S-metolachlor + mesotrione, was as high as 95 and 90% at 21 and 63 d after treatment (DAT), and mostly similar to the standard atrazine treatment. Residual control of common lambsquarters and wild buckwheat from pyroxasulfone was higher at 298 compared with 149 g ai ha−1 rate. Pyroxasulfone and other chloroacetamide herbicides (acetochlor or dimethenamid-P) applied alone failed to provide greater than 79, 70, and 54% residual control at 21, 35, and 63 DAT, respectively, of the weed species investigated. Residual weed control throughout the growing season was significantly improved with the addition of pendimethalin to pyroxasulfone (149 g ha−1), acetochlor, or dimethenamid-P when compared with any of the three herbicides applied alone. Kochia control by pyroxasulfone, acetochlor, or dimethenamid-P tank mixed with pendimethalin was as high as 94, 92, and 81% at 21, 35, and 63 DAT, respectively. Control of common lambsquarters with the addition of pendimethalin to pyroxasulfone or acetochlor was improved to 94, 89, and 81% at 21, 35, and 63 DAT, respectively. Similarly, wild buckwheat control with acetochlor plus pendimethalin was improved to 87, 85, and 82% at 21, 35, and 63 DAT, respectively. Consistent with the extended in-season (up to 9 wk) residual weed control, pyroxasulfone, acetochlor, or dimethenamid-P treatments when tank mixed with pendimethalin had higher corn yields compared with the herbicides applied alone. The investigation on residual herbicides that provide extended in-season weed control should be continued as an important aspect of glyphosate stewardship and to mitigate the occurrence of glyphosate-resistant weed populations in grower fields.Item Variable response of kochia [Kochia scoparia (L.) Schrad.] to auxinic herbicides dicamba and fluroxypyr in Montana(2015-09) Jha, Prashant; Kumar, Vipan; Lim, Charlemagne A.Herbicide-resistant kochia is an increasing concern for growers in the Northern Great Plains of United States and Canada. The objective of the research was to characterize the response of the three putative auxinic herbicide-resistant kochia inbreds (derived from accessions collected from wheat/ chemical fallow fields in northern Montana) to dicamba and fluroxypyr relative to a susceptible (SUS) inbred. A dicamba dose-response study indicated that the three putative resistant inbreds (Chot-01, Chot-02, and Chot-03) had R/S ratios of 1.3 to 6.1 based on the visible control response (I50 values), and R/S ratios of 1.5 to 6.8 based on the shoot dry weight response (GR50 values). Dose-response experiments with fluroxypyr determined I50 R/S ratios of 1.4 to 5.7 and GR50 R/S ratios of 1.6 to 4.0 for the three putative resistant inbreds. The selected inbreds showed variable symptomology (phenotype) in response to dicamba and fluroxypyr. Among the three inbreds, Chot-01 exhibited the least epinasty, stem curling/swelling, and chlorosis/necrosis symptoms, and was resistant to dicamba and fluroxypyr. Growers should diversify their weed management tools to manage further spread of auxinic or multiple herbicide-resistant kochia in the region.Item Crop water use and stage-specific crop coefficients for irrigated cotton in the mid-south, United States(2015-07) Kumar, Vipan; Udeigwe, Theophilus K.; Clawson, Ernest L.; Rohli, Robert; Miller, Donnie K.Regional variations in environmental conditions, cultivars, and management practices necessitate locally derived tools for crop water use estimation and irrigation scheduling. A study was conducted in northeast Louisiana (mid-south US) aimed at estimating daily crop evapotranspiration (ETc) and reference evapotranspiration (ETo) and thus, developing local crop coefficient (Kc) curves for irrigated upland cotton. ETc was determined using paired weighing lysimeters installed near the middle of a 1-ha cotton field and planted with cotton as in the rest of the surrounding field, while ETo was calculated using the Standardized Reference Evapotranspiration Equation (SREE) developed by the American Society of Civil Engineers (ASCE), using estimates of weather variables from a nearby standard reference weather station. Stage-specific Kc values averaged over 2 years were 0.42, 1.25 and 0.70 for initial, midseason, and end season stages of cotton, respectively. The initial-stage Kc value was approximately 26% lower than the Food and Agricultural Organization (FAO)-adjusted initial Kc value. The mid- and end-season Kc values obtained in the study were approximately 6% and 11% greater, respectively, than the FAO-adjusted Kc values for the corresponding stages. The observed differences among the local stage-specific Kc values (especially at initial growth stage of cotton) and the FAO-adjusted initial Kc values could be attributed to regional variations in environmental conditions, cultivars, and management practices. The ETc and Kc values obtained from this study provide research-based information for future studies and the development of Kc-based irrigation tools in this region.Item Effective Preemergence and Postemergence Herbicide Programs for Kochia Control(2015-03) Kumar, Vipan; Jha, PrashantField experiments were conducted in 2011 through 2013 at the MSU Southern Agricultural Research Center near Huntley, MT, to evaluate the effectiveness of various PRE and POST herbicide programs for kochia control in the absence of a crop. PRE herbicides labeled for corn, grain sorghum, soybean, wheat/barley, and/or in chemical fallow were applied at recommended field-use rates. Acetochlor + atrazine, S-metolachlor + atrazine + mesotrione, and sulfentrazone applied PRE provided ≥91% control of kochia at 12 wk after treatment (WAT). Metribuzin, metribuzin + linuron, and pyroxasulfone + atrazine PRE provided 82% control at 12 WAT. PRE control with acetochlor + flumetsulam + clopyralid, pyroxasulfone alone, and saflufenacil + 2,4-D was ≤23% at 12 WAT. Paraquat + atrazine, paraquat + linuron, and paraquat + metribuzin controlled kochia ≥98% at 5 WAT. POST control with bromoxynil + fluroxypyr, paraquat, tembotrione + atrazine, and topramezone + atrazine treatments averaged 84% at 5 WAT, and did not differ from glyphosate. Control with POST-applied bromoxynil + pyrasulfotole, dicamba, diflufenzopyr + dicamba + 2,4-D, saflufenacil, saflufenacil + 2,4-D, saflufenacil + linuron was 67 to 78% at 5 WAT. Because of the presence of kochia resistant to acetolactate synthase-inhibiting herbicides at the test site, cloransulam-methyl was not a viable option for kochia control. In a separate greenhouse study, kochia accessions showed differential response to the POST herbicides (labeled for corn or soybean) tested. Tembotrione + atrazine, topramezone + atrazine, lactofen, or fomesafen effectively controlled the glyphosate-resistant kochia accession tested. Growers should utilize these effective PRE- or POST-applied herbicide premixes or tank mixtures (multiple modes of action) to control herbicide-resistant kochia accessions in the field. PRE herbicides with 8 wk of soil-residual activity on kochia would be acceptable if crop competition were present; however, a follow-up herbicide application may be needed to obtain season-long kochia control in the absence of crop competition. Nomenclature: 2,4-D; acetochlor; atrazine; bromoxynil; carfentrazone-ethyl; clopyralid; cloransulam-methyl; dicamba; diflufenzopyr; flumetsulam; flumioxazin; fluroxypyr; fomesafen; glufosinate; glyphosate; isoxaflutole; lactofen; linuron; MCPA; mesotrione; metolachlor; paraquat; pyrasulfotole; pyroxasulfone; saflufenacil; sulfentrazone; tembotrione; thifensulfuron; tribenuron; topramezone; kochia, Kochia scoparia (L.) Schrad.