Physics

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The Physics department is committed to education and research in physics, the study of the fundamental universal laws that govern the behavior of matter and energy, and the exploration of the consequences and applications of those laws. Our department is widely known for its excellent teaching and student mentoring. Our department plays an important role in the university’s Core Curriculum. We have strong academic programs with several options for undergraduate physics majors, leading to the B.S. degree, as well as graduate curricula leading to the M.S. and Ph.D. degrees. Our research groups span a variety of fields within physics. Our principal concentrations are in Astrophysics, Relativity, Gravitation and Cosmology, Condensed Matter Physics, Lasers and Optics, Physics Education, Solar Physics, and the Space Science and Engineering Lab.

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    Two-photon directed evolution of green fluorescent proteins
    (2015-07) Stoltzfus, Caleb R.; Barnett, Lauren M.; Drobizhev, Mikhail; Wicks, Geoffrey; Mikhaylov, Alexander E.; Hughes, Thomas E.; Rebane, Aleksander
    Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon imaging in living tissues, including the brain. Here we demonstrate a technique for quantitatively screening the two-photon excited fluorescence (2PEF) efficiency and 2PA cross section of tens of thousands of mutant FPs expressed in E. coli colonies. We use this procedure to move EGFP through three rounds of two-photon directed evolution leading to new variants showing up to a 50% enhancement in peak 2PA cross section and brightness within the near-IR tissue transparency wavelength range.
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