College of Letters & Science

Permanent URI for this communityhttps://scholarworks.montana.edu/handle/1/37

The College of Letters and Science, the largest center for learning, teaching and research at Montana State University, offers students an excellent liberal arts and sciences education in nearly 50 majors, 25 minors and over 25 graduate degrees within the four areas of the humanities, natural sciences, mathematics and social sciences.

Browse

Filters

20151

Settings

Author: search.filters.author.Barnett, Lauren M.search.filters.applied.f.department: search.filters.department.Cell Biology & Neuroscience.

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Two-photon directed evolution of green fluorescent proteins
    (2015-07) Stoltzfus, Caleb R.; Barnett, Lauren M.; Drobizhev, Mikhail; Wicks, Geoffrey; Mikhaylov, Alexander E.; Hughes, Thomas E.; Rebane, Aleksander
    Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon imaging in living tissues, including the brain. Here we demonstrate a technique for quantitatively screening the two-photon excited fluorescence (2PEF) efficiency and 2PA cross section of tens of thousands of mutant FPs expressed in E. coli colonies. We use this procedure to move EGFP through three rounds of two-photon directed evolution leading to new variants showing up to a 50% enhancement in peak 2PA cross section and brightness within the near-IR tissue transparency wavelength range.
Copyright (c) 2002-2022, LYRASIS. All rights reserved.