Quantitative NMR metabolite profiling of methicillin-resistant and methicillin-susceptible Staphylococcus aureus discriminates between biofilm and planktonic phenotypes

dc.contributor.authorAmmons, Mary Cloud B.
dc.contributor.authorTripet, Brian P.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorKirker, Kelly R.
dc.contributor.authorGross, M. A.
dc.contributor.authorStanisich, Jessica J.
dc.contributor.authorCopie, Valerie
dc.date.accessioned2016-12-05T17:40:49Z
dc.date.available2016-12-05T17:40:49Z
dc.date.issued2013-06
dc.description.abstractWound bioburden in the form of colonizing biofilms is a major contributor to nonhealing wounds. Staphylococcus aureus is a Gram-positive, facultative anaerobe commonly found in chronic wounds; however, much remains unknown about the basic physiology of this opportunistic pathogen, especially with regard to the biofilm phenotype. Transcriptomic and proteomic analysis of S. aureus biofilms have suggested that S. aureus biofilms exhibit an altered metabolic state relative to the planktonic phenotype. Herein, comparisons of extracellular and intracellular metabolite profiles detected by 1H NMR were conducted for methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) S. aureus strains grown as biofilm and planktonic cultures. Principal component analysis distinguished the biofilm phenotype from the planktonic phenotype, and factor loadings analysis identified metabolites that contributed to the statistical separation of the biofilm from the planktonic phenotype, suggesting that key features distinguishing biofilm from planktonic growth include selective amino acid uptake, lipid catabolism, butanediol fermentation, and a shift in metabolism from energy production to assembly of cell-wall components and matrix deposition. These metabolite profiles provide a basis for the development of metabolite biomarkers that distinguish between biofilm and planktonic phenotypes in S. aureus and have the potential for improved diagnostic and therapeutic use in chronic wounds.en_US
dc.description.sponsorshipCenter for Biofilm Engineering at Montana State University for cultures of S. aureus 10943 and S. aureus 6538; NIH (3P20GM103394−05S1); parent grant (8P20GM103394−05); NIH (1KO1GM103821−01); NIH (1RO3AR060995-01A1); Howard Hughes Medical Institute (HHMI) Undergraduate Fellowship; NIH (1-S10RR13878−01) (S10RR026659-01A1)en_US
dc.identifier.citationAmmons MC, Tripet BP, Carlson RP, Kirker KR, Gross MA, Stanisich JJ, Copié V, "Quantitative NMR metabolite profiling of methicillin-resistant and methicillin-susceptible Staphylococcus aureus discriminates between biofilm and planktonic phenotypes," Journal of Proteome Research, June 2014, 13(6): 2973–85.en_US
dc.identifier.issn1535-3893
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12303
dc.titleQuantitative NMR metabolite profiling of methicillin-resistant and methicillin-susceptible Staphylococcus aureus discriminates between biofilm and planktonic phenotypesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2973en_US
mus.citation.extentlastpage2985en_US
mus.citation.issue6en_US
mus.citation.journaltitleJournal of Proteome Researchen_US
mus.citation.volume13en_US
mus.contributor.orcidAmmons, Mary Cloud B.|0000-0002-9717-0844en_US
mus.data.thumbpage10en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1021/pr500120cen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentBiological Sciences.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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