Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats

dc.contributor.authorDong, Yiran
dc.contributor.authorSanford, Robert A.
dc.contributor.authorInskeep, William P.
dc.contributor.authorSrivastava, Vaibhav
dc.contributor.authorBulone, Vincent
dc.contributor.authorFields, Christopher J.
dc.contributor.authorYau, Peter M.
dc.contributor.authorSivaguru, Mayandi
dc.contributor.authorAhren, Dag
dc.contributor.authorFouke, Kyle W.
dc.contributor.authorWeber, Joseph
dc.contributor.authorWerth, Charles R.
dc.contributor.authorCann, Isaac K.
dc.contributor.authorKeating, Kathleen M.
dc.contributor.authorKhetani, Radhika S.
dc.contributor.authorHernandez, Alvaro G.
dc.contributor.authorWright, Chris
dc.contributor.authorBand, Mark
dc.contributor.authorImai, Brian S.
dc.contributor.authorFried, Glenn A.
dc.contributor.authorFouke, Bruce W.
dc.description.abstractThe evolutionarily ancient Aquificales bacterium Sulfurihydrogenibium spp. dominates filamentous microbial mat communities in shallow, fast-flowing, and dysoxic hot-spring drainage systems around the world. In the present study, field observations of these fettuccini-like microbial mats at Mammoth Hot Springs in Yellowstone National Park are integrated with geology, geochemistry, hydrology, microscopy, and multi-omic molecular biology analyses. Strategic sampling of living filamentous mats along with the hot-spring CaCO3 (travertine) in which they are actively being entombed and fossilized has permitted the first direct linkage of Sulfurihydrogenibium spp. physiology and metabolism with the formation of distinct travertine streamer microbial biomarkers. Results indicate that, during chemoautotrophy and CO2 carbon fixation, the 87-98% Sulfurihydrogenibium-dominated mats utilize chaperons to facilitate enzyme stability and function. High-abundance transcripts and proteins for type IV pili and extracellular polymeric substances (EPSs) are consistent with their strong mucus-rich filaments tens of centimeters long that withstand hydrodynamic shear as they become encrusted by more than 5mm of travertine per day. Their primary energy source is the oxidation of reduced sulfur (e.g., sulfide, sulfur, or thiosulfate) and the simultaneous uptake of extremely low concentrations of dissolved O2 facilitated by bd-type cytochromes. The formation of elevated travertine ridges permits the Sulfurihydrogenibium-dominated mats to create a shallow platform from which to access low levels of dissolved oxygen at the virtual exclusion of other microorganisms. These ridged travertine streamer microbial biomarkers are well preserved and create a robust fossil record of microbial physiological and metabolic activities in modern and ancient hot-spring ecosystems.en_US
dc.identifier.citationDong, Yiran, Robert A. Sanford, William P. Inskeep, Vaibhav Srivastava, Vincent Bulone, Christopher J. Fields, Peter M. Yau, Mayandi Sivaguru, Dag Ahren, Kyle W. Fouke, Joseph Weber, Charles R. Werth, Isaac K. Cann, Kathleen M. Keating, Radhika S. Khetani, Alvaro G. Hernandez, Chris Wright, Mark Band, Brian S. Imai, Glenn A. Fried, and Bruce W. Fouke. "Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats." Astrobiology (April 2019). DOI:10.1089/ast.2018.1965.en_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.titlePhysiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Matsen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.relation.universityMontana State University - Bozemanen_US


Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
1 MB
Adobe Portable Document Format
Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats (PDF)
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
826 B
Item-specific license agreed upon to submission
Copyright (c) 2002-2022, LYRASIS. All rights reserved.