Impact of dilute acid pretreatment conditions on p-coumarate removal in diverse maize lines


Prior work has identified that lignins recovered from dilute acid-pretreated corn stover exhibit superior performance in phenol–formaldehyde resins used in wood adhesive applications when compared to diverse process-modified lignins derived from other sources. This improved performance is hypothesized to be due to the higher content of unsubstituted phenolic groups specifically p-coumarate lignin esters. In this work, a diverse set of corn stover samples are employed that exhibit diversity in p-coumarate content and total lignin content to explore the relationship between dilute acid pretreatment conditions, p-coumarate ester hydrolysis, xylan solubilization, and the resulting glucose enzymatic hydrolysis yields. The goal of this study is to identify pretreatment conditions that preserve a significant fraction of the p-coumarate esters while simultaneously achieving high enzymatic hydrolysis yields. Kinetic parameters for p-coumarate ester hydrolysis were quantified and pretreatment-biomass combinations were identified that result in glucose hydrolysis yields of more than 90% while retaining nearly 50 mg p-coumarate/g lignin.




Saulnier, Brian K., Thanaphong Phongpreecha, Sandip K. Singh, and David B. Hodge. “Impact of Dilute Acid Pretreatment Conditions on p-Coumarate Removal in Diverse Maize Lines.” Bioresource Technology 314 (October 2020): 123750. doi:10.1016/j.biortech.2020.123750.
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