Tables S1 and S2 [dataset]

Abstract

This dataset is associated with the following article: Gan, F., S. Zhang, N. C. Rockwell, S. S. Martin, J. C. Lagarias, and D. A. Bryant. “Extensive Remodeling of a Cyanobacterial Photosynthetic Apparatus in Far-Red Light." Science 345, no. 6202 (August 21, 2014): 1312-1317. doi:10.1126/science.1256963

Description

Table S1. Genes encoding subunits of Photosystem I (PS I), Photosystem II (PS II), and phycobilisomes (PBS) and related proteins in strain JSC-1. The table shows transcript ratios for the listed genes for cells grown in far-red light for 24 hours compared to transcript levels for cells grown in white light. The table also shows those proteins that were identified by tryptic peptide LC-MS/MS analysis in gradient fractions containing PS I, PS II, and PBS from cells grown in red light and far-red light. An “unused peptide” score of 1.3 indicates at least 95% confidence for a positive identification, and a score greater than 2.0 indicates a 99% confidence for a positive identification. The pink (PS I), green (PS II) and blue (PBS) shading identifies the genes (proteins) for core components that are encoded in the 21-gene photosynthesis cluster shown in Fig. S2. Phycobiliproteins were low-abundance contaminants of fractions #1 and #2 containing PS I and PS II or PS I trimers (see Fig. 4). The composition data for PBS are more accurately reflected from the data for isolated PBS from cells grown in 645-nm light or 710-nm light. Table S2. Complete transcription profiling data for transcripts for strain JSC-1 cells grown in white light and far-red light. Cells were grown in white light to an OD750 nm = 0.3 and were then transferred to far-red light for 24 hours prior to isolation of RNA from each condition. For additional details, see SOM, Materials and Methods.

Keywords

Citation

Gan F, Zhang S, Rockwell NC, Martin SS, Lagarias JC, Bryant DA (2014) Tables S1 and S2 [dataset]. Montana State University ScholarWorks. http://scholarworks.montana.edu/handle/1/8727
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