Scholarly Work - Plant Sciences & Plant Pathology
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8870
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Item QTL mapping reveals malt barley quality improvement in two dryland environments associated with extended grain fill and seminal root traits(Wiley, 2024-05) Williams, Jessica L.; Lamb, Peggy F.; Lutgen, Greg; Lachowiec, Jennifer; Cook, Jason P.; Jensen, Joseph; Bourgault, Maryse; Sherman, Jamie D.To achieve malt grade and receive full price, barley (Hordeum vulgare L.) crops must meet standards for certain quality traits including percent plump and protein. Terminal drought stress reduces quality and is projected to worsen in barley cultivation areas, underscoring the need for varieties that maintain good malt production with unreliable precipitation. The stay-green trait extends the grain fill phase between heading and maturity and has been linked to stable quality under dry conditions. However, this relationship can be inconsistent and is not well understood. To effectively leverage a longer grain fill phenotype for drought adaptation, a better grasp of its genetics and environmental interaction is needed. Stay-green root system differences have been observed and could be at play. We performed correlation and quantitative trait locus (QTL) analysis on grain fill duration, grain quality, and seminal root traits using a recombinant inbred line (RIL) population segregating for stay-green. Agronomic data were collected in four field trials at two distinct semiarid locations, and roots were measured in a greenhouse assay. Earlier heading and later maturity led to improved quality in both locations and more consistent quality between locations. Earlier heading had a greater influence on quality in the drier environment, while later maturity was more impactful in the less dry environment. We observed co-locations of seminal root trait QTLs with grain fill duration and grain quality. These QTLs lay the groundwork for further investigation into root phenotypes associated with stay-green and the deployment of these traits in breeding for drought adaptation.Item Evaluation of wheat stem sawfly‐resistant solid stem Qss.msub‐3BL alleles in hard red winter wheat(Wiley, 2023-01) Wong, Mei Ling; Bruckner, Philip L.; Berg, Jim E.; Lamb, Peggy F.; Hofland, Megan L.; Caron, Christopher G.; Heo, Hwa‐Young; Blake, Nancy K.; Weaver, David K.; Cook, Jason P.Host plant resistance provided by solid stems has been the most effective means for mitigating wheat stem sawfly (WSS) (Cephus cinctus Norton) damage in spring and winter wheat (Triticum aestivum L.). The solid stem trait originates from the spring wheat cultivar “Rescue” and is associated with a quantitative trait locus allele Qss.msub-3BL.b that explains the majority of the variation for stem solidness. Recently, a new Qss.msub-3BL solid stem allele, designated Qss.msub-3BL.c, was identified in the spring wheat cultivar “Conan”. It produces a solid stem phenotype early in plant development but dissipates during plant growth. The Qss.msub-3BL.c allele provides effective WSS resistance in spring wheat but has not been tested in winter wheat. To examine if the Qss.msub-3BL.c allele provides adequate WSS resistance in winter wheat, near-isogenic lines (NILs) were developed using marker-assisted backcrossing. This enabled comparisons between the hollow stem Qss.msub-3BL.a, solid stem Qss.msub-3BL.b and solid stem Qss.msub-3BL.c alleles for stem solidness, WSS resistance, and agronomic traits in Montana growing environments. Compared to the hollow stem allele, the NILs with the Qss.msub-3BL.c allele increased stem solidness and reduced WSS stem cutting. However, the Qss.msub-3BL.c allele resulted in lower solid stem scores and greater WSS stem cutting compared to the Qss.msub-3BL.b allele. Overall, these findings indicate that the Qss.msub-3BL.c allele failed to provide sufficient WSS resistance in the winter wheat backgrounds tested in this study.Item Registration of ‘Lustre’ durum wheat(Wiley, 2022-08) Hogg, Andrew C.; Carr, Patrick; Eberly, Jed; Chen, Chengci; Kowatch‐Carlson, Calla; Crutcher, Frankie; Lamb, Peggy F.; McNamara, Kyla; Haney, Eleri; Kephart, Ken D.‘Lustre’ (Reg. no. CV-1193, PI 695072) is a spring durum wheat [Triticum turgidum L. ssp. durum (Desf.)] developed by the Montana Agricultural Experiment Station and released in 2020. Lustre was bred using the single seed descent method and was selected for its yield performance under dryland conditions across Montana, low grain Cd accumulation, good pasta firmness, high grain protein, high yellow semolina color, and low semolina ash. Lustre performs well in both the north central and northeast regions of Montana, where most Montana durum is produced and intended for pasta production. Lustre has similar stripe rust tolerance and susceptibility as top-grown durum cultivars in the state with susceptibility at the seedling stage and high-temperature adult-plant resistance. Lustre is moderately susceptible to Fusarium head blight like other durum cultivars. Lustre is resistant to the predominant races of stem and leaf rust and is moderately tolerant to fungal leaf spot complex. Lustre is approximately 89 cm tall, with a yellow green color and a heading date 1 d later than the cultivar ‘Mountrail’. Lustre has an erect flag leaf and an erect tapering head having white glumes and awns.Item Relationships between roots, the stay‐green phenotype, and agronomic performance in barley and wheat grown in semi‐arid conditions(Wiley, 2022-01) Williams, Jessica L.; Sherman, Jamie D.; Lamb, Peggy F.; Cook, Jason; Lachowiec, Jennifer A.; Bourgault, MaryseStay-green is a phenotype that crop breeders could use to improve drought adaptation. It increases the duration of grain fill in several species including barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.), maintaining yield in semi-arid conditions. Evidence from controlled environment experiments suggests a connection between stay-green and root systems. These belowground structures are understudied and thus represent opportunity for crop improvement if relationships to agronomics can be understood. Minirhizotrons facilitate study of these relationships by allowing repeated nondestructive root measurements in field conditions. However, this is time-consuming, and proxies would be useful for increasing throughput capacity of root research. Here we present results from field trials with minirhizotrons in a semi-arid environment, as well as greenhouse seedling assays conducted on stay-green and non-stay-green barley and wheat lines. In barley, stay-green and greater yield were primarily associated with greater deep root length and delayed root senescence, whereas in wheat, yield was most strongly correlated with total root length, and root system differences for stay-green were not as apparent. We speculate that the physiology of stay-green is different between these two species, and that barley may use a more efficient root system to withstand drought whereas wheat relies on a larger one. Several seedling traits related consistently to field root traits, but correlation directions were often opposite between barley and wheat. The connections between traits presented here could be useful for breeders seeking to improve crop adaptation to drought, but more genotypes and environments will need to be tested.Item Registration of ‘StandClear CLP’ hard red winter wheat(2020-06) Berg, Jim E.; Kephart, Ken D.; Lamb, Peggy F.; Davis, Edward S.; Eberly, Jed O.; Miller, John H.; Chen, Chengci; Pradhan, G. P.; Torrion, Jessica A.; Ramsfield, Ron; Smith, Vincent H.; Nash, Deanna L.; Holen, Doug L.; Cook, Jason P.; Gale, Sam; Jin, Yue; Chen, X.; Bruckner, Phil L.‘StandClear CLP’ (Reg. no. CV-1162, PI 693236) hard red winter (HRW) wheat (Triticum aestivum L.) was developed and released by the Montana Agricultural Experiment Station and exclusively licensed to Loveland Products, Inc., in 2020. StandClear CLP is a two-gene Clearfield, semisolid-stem wheat intended for use with the selective imidazolinone (IMI) herbicide imazamox. StandClear CLP resulted from a cross of MTS0531 to an IMI herbicide tolerant F1 plant from a population segregating for two acetohydroxyacid synthase (AHAS) genes [TaAHAS1D and TaAHAS1B]. Original herbicide tolerance donors were IMI ‘Fidel’ (TX12588*4/FS2, BASF) for allele TaAHAS1D via descended experimental lines MTCL0309 and MTCL0510, and proprietary hard red spring wheat line CDC Teal 11A (BASF Corporation) for allele TaAHAS1B. StandClear CLP was selected as a F6:7 headrow in 2014 following multiple cycles of phenotypic mass selection for IMI herbicide tolerance and stem solidness. StandClear CLP was tested under the experimental number MTCS1601 from 2016 to 2019 in Montana for field performance, herbicide tolerance, and end-use quality. StandClear CLP is a high-yielding, Clearfield HRW wheat cultivar with intermediate stem solidness, moderate host plant resistance to wheat stem sawfly, and acceptable milling and baking quality.Item Warm-Season Forage Options in Northern Dryland Regions(2020-06) Carr, Patrick M.; Boss, Darrin L.; Chen, Chengci; Dafoe, Julia M.; Eberly, Jed O.; Fordyce, Simon; Hydner, Roger M.; Fryer, Heather K.; Lachowiec, Jennifer A.; Lamb, Peggy F.; McVay, Kent A.; Khan, Qasim A.; Miller, Perry R.; Miller, Zachariah J.; Torrion, Jessica A.Rotating summer fallow with wheat (Triticum spp.) is done in dryland grain farming at upper latitudes to stabilize yields over time and to prevent crop failure. However, summer fallow is costly since weeds must be controlled and crops are not grown. Replacing summer fallow with grain crops can generate low economic returns. Previous research indicated that annual cool‐season forages can be substituted for summer fallow in dryland cropping systems. Our objective was to determine if annual warm‐season species were suited for forage production in monocultures and polycultures in the U.S. northern Great Plains. Dry matter (DM) production by 20 warm‐ and cool‐season crop monocultures and 4 polycultures was determined across six environments during 2016, and by 25 warm‐ and cool‐season crop monocultures and polycultures across four environments from 2016 through 2018. Maize (Zea mays L.) monoculture produced forage DM in amounts equal to, or greater than, those produced by other warm‐ and cool‐season crop treatments (P < 0.05). Maize DM production averaged 2.5 to 5.7 Mg ha−1, depending on the study and environment. Sorghum (Sorghum bicolor L.), foxtail millet [Setaria italica (L.) P. Beauv.] and sunflower (Helianthus annuus L.) also produced relatively large amounts of forage DM. Polycultures failed to produce more DM than monocultures consistently (P > 0.40). These results indicate that maize and other warm‐season crops are adapted for dryland forage production in cool regions at upper latitudes. Additional research is needed to determine the impacts of annual warm‐season forages on grain yield in a forage‐wheat crop sequence.Item Registration of ‘Dagmar’ hard red spring wheat(2020-02) Heo, Hwa-Young; Lanning, Susan P.; Lamb, Peggy F.; Nash, Deanna L.; Wichman, David M.; Eberly, Jed O.; Carr, P.; Kephart, Ken D.; Stougaard, Robert N.; Torrion, Jessica A.; Miller, J.; Chen, Chengci; Holen, Doug L.; Blake, Nancy K.; Talbert, Luther E.‘Dagmar’ hard red spring wheat (Triticum aestivum L.) (Reg. no. CV‐1158, PI 690450) was released by the Montana Agricultural Experiment Station because of its excellent yield potential in dryland areas of Montana, solid stems, and superior end‐use quality. Dagmar was a selection from the cross MT1133/MT1148 and was tested as experimental line MT1621. Dagmar has similar grain yield potential to ‘Vida’, the most widely grown cultivar in Montana. Stems of Dagmar are more solid than those of Vida, suggesting increased resistance to the wheat stem sawfly (Cephus cinctus Norton). Dagmar has higher grain protein and stronger gluten than Vida. Thus, Dagmar should be useful in Montana and adjoining states facing drought and wheat stem sawfly pressure.Item Characterization of resistance to Cephus cinctus Norton (Hymenoptera: Cephidae) in barley germplasm(2018-04) Varella, Andrea C.; Talbert, Luther E.; Achhami, Buddhi B.; Blake, Nancy K.; Hofland, Megan L.; Sherman, Jamie D.; Lamb, Peggy F.; Reddy, Gadi V. P.; Weaver, David K.Most barley cultivars have some degree of resistance to the wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae). Damage caused by WSS is currently observed in fields of barley grown in the Northern Great Plains, but the impact of WSS damage among cultivars due to genetic differences within the barley germplasm is not known. Specifically, little is known about the mechanisms underlying WSS resistance in barley. We characterized WSS resistance in a subset of the spring barley CAP (Coordinated Agricultural Project) germplasm panel containing 193 current and historically important breeding lines from six North American breeding programs. Panel lines were grown in WSS infested fields for two consecutive years. Lines were characterized for stem solidness, stem cutting, WSS infestation (antixenosis), larval mortality (antibiosis), and parasitism (indirect plant defense). Variation in resistance to WSS in barley was compared to observations made for solid-stemmed resistant and hollow-stemmed susceptible wheat lines. Results indicate that both antibiosis and antixenosis are involved in the resistance of barley to the WSS, but antibiosis seems to be more prevalent. Almost all of the barley lines had greater larval mortality than the hollow-stemmed wheat lines, and only a few barley lines had mortality as low as that observed in the solid-stemmed wheat line. Since barley lines lack solid stems, it is apparent that barley has a different form of antibiosis. Our results provide information for use of barley in rotation to control the WSS and may provide a basis for identification of new approaches for improving WSS resistance in wheat.Item Characterization of resistance to the wheat stem sawfly in spring wheat landrace accessions from targeted geographic regions of the world(2017-07) Varella, Andrea C.; Weaver, David K.; Cook, Jason P.; Blake, Nancy K.; Hofland, Megan L.; Lamb, Peggy F.; Talbert, Luther E.Plant landraces have long been recognized as potential gene pools for biotic and abiotic stress-related genes. This research used spring wheat landrace accessions to identify new sources of resistance to the wheat stem sawfly (WSS) (Cephus cinctus Norton), an important insect pest of wheat in the northern Great Plains of North America. Screening efforts targeted 1409 accessions from six geographical areas of the world where other species of grain sawflies are endemic or where a high frequency of accessions possesses the resistance characteristic of solid stems. Resistance was observed in approximately 14% of accessions. Half of the lines displayed both antixenosis and antibiosis types of resistance. Among the resistant accessions, 41% had solid or semi-solid stems. Molecular genetic screening for haplotypes at the solid stem QTL, Qss.msub.3BL, showed that 15% of lines shared the haplotype derived from \'S-615\', the original donor of the solid stem trait to North American germplasm. Other haplotypes associated with solid stems were also observed. Haplotype diversity was greater in the center of origin of wheat. Evaluation of a representative set of resistant landrace accessions in replicated field trials at four locations over a three year period identified accessions with potential genes for reduced WSS infestation, increased WSS mortality, and increased indirect defense via parasitoids. Exploitation of distinct types of plant defense will expand the genetic diversity for WSS resistance currently present in elite breeding lines.Item Registration of 'Northern' Hard Red Winter Wheat(2016-05) Berg, Jim E.; Lamb, Peggy F.; Miller, John H.; Wichman, David M.; Kephart, Ken D.; Stougaard, Robert N.; Pradhan, G. P.; Nash, Deanna L.; Grey, William E.; Gettel, D.; Gale, Sam; Jin, Yue; Kolmer, J. A.; Chen, X.; Bai, G.; Murray, T. D.; Bruckner, Phil L.Northern' (Reg. No. CV-1114, PI 676026) hard red winter wheat (Triticum aestivum L.) was developed and released by the Montana Agricultural Experiment Station in 2015. Northern was derived from a composite of two crosses, MT9982//MTW0072/NW97151 and MTW0047//MTW0072/NW97151. Northern was developed using a modified bulk breeding method and selected as an F-5:7 headrow. Northern was tested under the experimental number MT0978 in Montana yield trials from 2009 to 2015. Like predominant cultivar Yellowstone, Northern is a high-yielding, winter-hardy hard red winter wheat cultivar with medium to late maturity, medium to high grain protein, and acceptable milling and baking quality. Northern was released for its excellent performance in winter wheat production environments of north-central Montana, reduced plant height, and improved grain volume weight and resistance to stem rust (caused by Puccinia graminis Pers.: Pers. f. sp. tritici Eriks. & E. Henn.) relative to Yellowstone.